Genetic interaction (GI) analysis is a powerful genetic strategy that analyzes the fitness and phenotypes of single- and double-gene mutant cells in order to dissect the epistatic interactions between genes, categorize genes into biological pathways, and characterize genes of unknown function. GI analysis has been extensively employed in model organisms for foundational, systems-level assessment of the epistatic interactions between genes. More recently, GI analysis has been applied to microbial pathogens and has been instrumental for the study of clinically important infectious organisms. Here, we review recent advances in systems-level GI analysis of diverse microbial pathogens, including bacterial and fungal species. We focus on important applications of GI analysis across pathogens, including GI analysis as a means to decipher complex genetic networks regulating microbial virulence, antimicrobial drug resistance and host-pathogen dynamics, and GI analysis as an approach to uncover novel targets for combination antimicrobial therapeutics. Together, this review bridges our understanding of GI analysis and complex genetic networks, with applications to diverse microbial pathogens, to further our understanding of virulence, the use of antimicrobial therapeutics and host-pathogen interactions. .

中文翻译：

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微生物病原体的遗传相互作用分析：揭示发病机制、抗菌药物敏感性和宿主相互作用的网络。

遗传相互作用（GI）分析是一种强大的遗传策略，可分析单基因和双基因突变细胞的适应性和表型，以剖析基因之间的上位相互作用，将基因分类为生物途径，并表征未知功能的基因。GI 分析已广泛应用于模型生物体，用于对基因之间的上位相互作用进行基础、系统级评估。最近，胃肠道分析已应用于微生物病原体，并有助于研究临床上重要的感染性生物体。在这里，我们回顾了多种微生物病原体（包括细菌和真菌物种）系统级胃肠道分析的最新进展。我们专注于胃肠道分析在病原体中的重要应用，包括胃肠道分析作为破译调节微生物毒力、抗菌药物耐药性和宿主-病原体动态的复杂遗传网络的手段，以及胃肠道分析作为发现联合抗菌治疗新靶点的方法。总之，这篇综述架起了我们对胃肠道分析和复杂遗传网络的理解，并将其应用于不同的微生物病原体，以进一步我们对毒力、抗菌疗法的使用和宿主-病原体相互作用的理解。。