In the present study, we evaluated the antibacterial activity of Citrus reticulata (C. reticulata) essential oil-incorporated gelatin film solution against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The minimum inhibitory concentration (MIC) of C. reticulata essential oil (CrEO) on E. coli and S. aureus were found to be 10.1 and 9.1 mg mL-1, respectively, using spectrophotometric microdilution technique. The antimicrobial effect of CrEO alone and incorporated gelatin-based film solutions (CrEO-F) against these pathogens were determined by the disc diffusion method. While the inhibition zones of CrEO were 2.4 mm and 10.05 mm, CrEO-F was reported as 1.5 and 7.8 mm against E. coli and S. aureus, respectively. These findings demonstrated that the CrEO can compete with coating agents with antimicrobial additives and it can find a place in the application areas. Besides, the chemical composition of the CrEO was determined by GC-MS.

中文翻译：

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柑桔香精油的评价：明胶对大肠杆菌和金黄色葡萄球菌的化学组成和抗菌效果。

在本研究中，我们评估了柑桔柑桔（C. reticulata）精油结合明胶薄膜溶液对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌活性。使用分光光度微量稀释技术，发现网状隐孢子虫精油（CrEO）对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度（MIC）分别为10.1和9.1 mg mL-1。通过圆盘扩散法确定了单独的CrEO和掺入的明胶基薄膜溶液（CrEO-F）对这些病原体的抗菌作用。虽然CrEO的抑制区为2.4 mm和10.05 mm，但据报道CrEO-F对大肠杆菌和金黄色葡萄球菌的抑制区分别为1.5 mm和7.8 mm。这些发现表明，CrEO可以与带有抗菌添加剂的涂层剂竞争，并且可以在应用领域中找到一席之地。此外，CrEO的化学组成通过GC-MS测定。