A nitrous oxide (N2O)-consuming bacterium isolated from farmland soil actively consumed N2O under high pH conditions. An acetylene inhibition assay did not show the denitrification of N2 to N2O by this bacterium. When N2O was injected as the only nitrogen source, this bacterium did not assimilate N2O. A polymerase chain reaction demonstrated that this bacterium did not have the typical nosZ gene. This bacterium belonged to Chitinophagaceae, but did not belong to known families that include bacteria with the atypical nosZ. This is the first study to show that a non-denitrifier actively reduces N2O, even under high pH conditions.

中文翻译：

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从土壤中分离出的一种未知的非反硝化细菌在高 pH 条件下主动减少一氧化二氮

从农田土壤中分离出的消耗一氧化二氮 (N2O) 的细菌在高 pH 条件下积极消耗 N2O。乙炔抑制试验未显示该细菌将 N2 反硝化为 N2O。当 N2O 作为唯一的氮源被注入时，这种细菌不会同化 N2O。聚合酶链反应证明这种细菌没有典型的 nosZ 基因。这种细菌属于几丁质噬菌科，但不属于包括非典型 nosZ 细菌的已知家族。这是第一项表明非反硝化剂即使在高 pH 条件下也能主动减少 N2O 的研究。