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Enzymatic construction of metal-mediated nucleic acid base pairs.
Metallomics ( IF 3.4 ) Pub Date : 2021-04-21 , DOI: 10.1093/mtomcs/mfab016
Marie Flamme 1, 2, 3 , Chiara Figazzolo 1, 2, 4 , Gilles Gasser 3 , Marcel Hollenstein 1
Affiliation  

Artificial metal base pairs have become increasingly important in nucleic acids chemistry due to their high thermal stability, water solubility, orthogonality to natural base pairs, and low cost of production. These interesting properties combined with ease of chemical and enzymatic synthesis have prompted their use in several practical applications, including the construction of nanomolecular devices, ions sensors, and metal nanowires. Chemical synthesis of metal base pairs is highly efficient and enables the rapid screening of novel metal base pair candidates. However, chemical synthesis is limited to rather short oligonucleotides and requires rather important synthetic efforts. Herein, we discuss recent progress made for the enzymatic construction of metal base pairs that can alleviate some of these limitations. First, we highlight the possibility of generating metal base pairs using canonical nucleotides and then describe how modified nucleotides can be used in this context. We also provide a description of the main analytical techniques used for the analysis of the nature and the formation of metal base pairs together with relevant examples of their applications.

中文翻译:

金属介导的核酸碱基对的酶促构建。

人造金属碱基对由于其高的热稳定性,水溶性,与天然碱基对的正交性以及较低的生产成本而在核酸化学中变得越来越重要。这些有趣的特性以及易于化学和酶促合成的特性促使它们在多种实际应用中使用,包括构造纳米分子设备,离子传感器和金属纳米线。金属碱基对的化学合成是高效的,并且能够快速筛选新型金属碱基对候选物。然而,化学合成限于相当短的寡核苷酸,并且需要相当重要的合成努力。本文中,我们讨论了可减轻这些局限性的金属碱基对酶促构建的最新进展。第一的,我们强调了使用规范核苷酸生成金属碱基对的可能性,然后描述了如何在这种情况下使用修饰的核苷酸。我们还提供了用于分析金属碱基对的性质和形成的主要分析技术的说明,以及它们的相关应用实例。
更新日期:2021-04-21
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