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A Simple Bioanalytical Method for Simultaneous Estimation of Amlodipine and Celecoxib in Rat Plasma by High Performance Liquid Chromatography
Journal of Chromatographic Science ( IF 1.3 ) Pub Date : 2021-04-07 , DOI: 10.1093/chromsci/bmab046
M U Maneesh, Syed Sagheer Ahmed, T Yunus Pasha, B Ramesh, Manish Majumder

A simple, precise, rapid and accurate UFLC method has been developed with due validation for the simultaneous estimation of Amlodipine besylate and Celecoxib in rat plasma. The separation has been taken place by C18 Eclipse plus column at 1ml/min flow rate. The mobile phase comprises of 20 mM sodium acetate buffer of pH 4.5 adjusted with glacial acetic acid and methanol (30:70% v/v). The effluents were monitored at 228 nm with a total run time of 15min. The retention time of Amlodipine besylate and celecoxib were found to be 7.69 min and 10.69 min respectively. The extraction of drugs have been achieved by protein precipitation technique with methanol as a solvent. The detection concentration was linear over 60–420 ng/ml for Amlodipine besylate and 600-4200 ng/ml for Celecoxib. Regression equation of Amlodipine besylate and Celecoxib were found to be y = 30.996x + 520.29 & y = 39.722x + 23706 with regression coefficient 0.9944 & 0.9941 respectively using unweighted and weighted linear regression with a weighting factor of 1/x0, 1/x, 1/✓x and 1/x2. The percentage recoveries were found to be 88.52±1.276 to 93.06±2.872 for Amlodipine & 89.40±0.728 to 94.05±0.221 for Celecoxib. This liquid chromatography method was extensively validated for linearity, accuracy precision, and stability studies.

中文翻译:

高效液相色谱法同时测定大鼠血浆中氨氯地平和塞来昔布的简单生物分析方法

已开发出一种简单、精确、快速且准确的 UFLC 方法,并经过适当验证,可同时测定大鼠血浆中苯磺酸氨氯地平和塞来昔布的含量。通过 C18 Eclipse plus 柱以 1ml/min 的流速进行分离。流动相包含用冰醋酸和甲醇 (30:70% v/v) 调节的 pH 4.5 的 20 mM 乙酸钠缓冲液。在 228 nm 处监测流出物,总运行时间为 15 分钟。苯磺酸氨氯地平和塞来昔布的保留时间分别为 7.69 分钟和 10.69 分钟。以甲醇为溶剂,通过蛋白质沉淀技术实现了药物的提取。苯磺酸氨氯地平的检测浓度线性超过 60-420 ng/ml,塞来昔布的检测浓度线性超过 600-4200 ng/ml。使用加权因子为 1/x0、1/x、 1/✓x 和 1/x2。氨氯地平的回收率百分比为 88.52±1.276 至 93.06±2.872,塞来昔布的回收率百分比为 89.40±0.728 至 94.05±0.221。这种液相色谱方法在线性、准确度、精密度和稳定性研究方面得到了广泛验证。塞来昔布为 221。这种液相色谱方法在线性、准确度、精密度和稳定性研究方面得到了广泛验证。塞来昔布为 221。这种液相色谱方法在线性、准确度、精密度和稳定性研究方面得到了广泛验证。
更新日期:2021-04-07
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