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Validation of the Eclipse Farm 4G & COMET for Detection of Antibiotics in Raw Bovine Milk: AOAC Performance Tested MethodSM 022101.
Journal of AOAC INTERNATIONAL ( IF 1.6 ) Pub Date : 2021-04-28 , DOI: 10.1093/jaoacint/qsab061
Pedro Razquin 1 , David Sanz 1 , Adrián Marco 1 , Vanesa Carrascón 1 , Sofía Andaluz 1 , Lourdes Soler 1 , Alejandra Antón 1 , Luis Mata 1
Affiliation  

BACKGROUND The Eclipse Farm 4 G test coupled to the COMET device allows for automatic and easy screening of a broad range of antimicrobials in raw bovine milk. All results obtained with this system are available in real-time through a smartphone application (App) and in the Test4all cloud platform. OBJECTIVE The objective of this study was to validate Eclipse Farm 4 G & COMET for the screening of antimicrobials in raw bovine milk according to AOAC Performance Tested MethodSM procedures. METHOD The test is based on the inhibition of microbial growth of Geobacillus stearothermophilus in presence of antimicrobials. When sample tubes are incubated in the absence of antibiotics, spores germinate and cells grow changing medium color from purple to yellow (negative result). The presence of antimicrobials in the milk sample hinders bacterial growth preventing the tubes from changing color from purple to yellow (positive result). The COMET device automatically integrates all assay steps (incubation, time control, and results interpretation) and communications between device, smartphone, and cloud. RESULTS LODs and detection capabilities (CCβs) were confirmed to be at or below the EU-MRL for most of the evaluated molecules representing the main families of antimicrobials (penicillins, cephalosporins, tetracyclines, sulfonamides, macrolides, and aminoglycosides). False positive rate and the effect of potentially interfering substances showed sufficient selectivity/specificity. Eclipse Farm 4 G & COMET was shown to be robust, consistent and stable during shelf life. CONCLUSIONS The Eclipse Farm 4 G & COMET system has been shown to be suitable for screening antibiotics in raw bovine milk. HIGHLIGHTS The assay is an automatic and easy system for the detection of a broad range of antibiotics in raw bovine milk consistent with EU-MRLs and can provide results anytime and anywhere through a smartphone App and a cloud platform.

中文翻译:

Eclipse Farm 4G和COMET验证用于检测生牛乳中的抗生素:通过AOAC性能测试的MethodSM 022101。

背景技术与COMET设备耦合的Eclipse Farm 4 G测试允许对生牛乳中的多种抗菌素进行自动,简便的筛选。通过该系统获得的所有结果都可以通过智能手机应用程序(App)和Test4all云平台实时获得。目的本研究的目的是根据AOAC Performance Tested MethodSM程序验证Eclipse Farm 4 G和COMET筛选生牛乳中的抗菌剂。方法该试验基于在存在抗菌剂的情况下对嗜热嗜热地芽孢杆菌微生物生长的抑制作用。当在没有抗生素的情况下孵育样品管时,孢子发芽,细胞生长,培养基颜色从紫色变为黄色(阴性结果)。牛奶样品中存在抗微生物剂会阻碍细菌的生长,从而阻止试管的颜色从紫色变为黄色(阳性结果)。COMET设备自动集成所有测定步骤(孵育,时间控制和结果解释)以及设备,智能手机和云之间的通信。结果对于代表抗菌素主要家族的大多数被评估分子(青霉素,头孢菌素,四环素,磺酰胺,大环内酯和氨基糖苷),LOD和检测能力(CCβ)均在EU-MRL或以下。假阳性率和潜在干扰物质的影响显示出足够的选择性/特异性。事实证明,Eclipse Farm 4 G&COMET在货架期内稳定,稳定且稳定。结论Eclipse Farm 4 G&事实证明,COMET系统适用于筛查生牛乳中的抗生素。要点该测定法是一种自动简便的系统,用于检测与EU-MRL一致的生牛乳中的多种抗生素,并可以通过智能手机应用程序和云平台随时随地提供结果。
更新日期:2021-04-28
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