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RNA sequencing and proteomic profiling reveal different alterations by dietary methylmercury in the hippocampal transcriptome and proteome in BALB/c mice.
Metallomics ( IF 3.4 ) Pub Date : 2021-04-23 , DOI: 10.1093/mtomcs/mfab022
Ragnhild Marie Mellingen 1, 2 , Lene Secher Myrmel 1 , Kai Kristoffer Lie 1 , Josef Daniel Rasinger 1 , Lise Madsen 1, 3 , Ole Jakob Nøstbakken 1
Affiliation  

Methylmercury (MeHg) is a highly neurotoxic form of mercury present in seafood. Here we recorded and compared proteomics and transcriptomics changes in hippocampus of male BALB/c mice exposed to two doses of MeHg. Mice were fed diets spiked with 0.28 mg MeHg kg-1, 5 mg MeHg kg-1 or an unspiked control diet for 77 days. Total mercury (Hg) content was significantly (p < 0.05) increased in brain tissue of both MeHg-exposed groups (18 ± 2 mg Hg kg-1 and 0.56 ± 0.06 mg Hg kg-1). Hippocampal protein and RNA expression levels were significantly altered both in tissues from mice receiving a low dose MeHg (20 proteins/294 RNA transcripts) or a high dose MeHg (61 proteins/876 RNA transcripts). The majority, but not all the differentially expressed features in hippocampus were dose dependent. The combined use of transcriptomic and proteomic profiling data provided insight on the influence of MeHg on neurotoxicity, energy metabolism and oxidative stress through several regulated features and pathways, including RXR function and superoxide radicals degradation.

中文翻译:

RNA测序和蛋白质组学分析揭示了饮食中甲基汞在BALB / c小鼠海马转录组和蛋白质组中的变化。

甲基汞(MeHg)是海鲜中存在的高度神经毒性的汞形式。在这里,我们记录并比较了暴露于两次MeHg剂量的雄性BALB / c小鼠海马中的蛋白质组学和转录组学变化。给小鼠饲喂掺有0.28 mg MeHg kg-1、5 mg MeHg kg-1或未掺餐的对照饮食的饮食77天。暴露于MeHg的两个组的脑组织中总汞(Hg)含量均显着增加(p <0.05)(18±2 mg Hg kg-1和0.56±0.06 mg Hg kg-1)。接受低剂量MeHg(20种蛋白质/ 294 RNA转录本)或高剂量MeHg(61种蛋白质/ 876 RNA转录本)的小鼠组织中的海马蛋白和RNA表达水平均发生了显着变化。海马中大多数(但不是全部)差异表达的特征是剂量依赖性的。
更新日期:2021-04-23
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