Many of the bioactive substances used in pharmaceutical industry are easily affected by temperature, light and oxygen, and are easily degraded during storage and processing, and exhibit poor adsorption properties during digestion, which limits their direct use. Microalgae are rich in oils which have antimicrobial properties and antioxidants that attract attention in both food and pharmaceutical sectors in recent years. Studies to encapsulate bioactive compound-rich microalgae oils with nanotechnological approaches to improve the physical and chemical stability are relatively new, and it is promising to apply these approaches for pharmaceutical purposes. In this study, cytotoxic effects of oil extracts of Botryococcus braunii and Microcystis aeruginosa and their oil-loaded nanoparticles on L929 cell line, PC-3 prostate cell line, SHSY-5Y neuroblastoma cell line and AGS gastric adenocarcinoma cell line were investigated. The obtained extracts were found to have no cytotoxic effect on L929 cells. However, they showed cytotoxic effect on cancer cells. As for the nanoparticles; a gradual release was determined and the stability of the nanoparticle structure was shown. In the light of obtained findings, it was considered that nanoparticles produced with oil extracts of microalgae which have bioactive substances, have potential to be evaluated especially in pharmaceutical and cosmetic fields.

中文翻译：

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微藻油和负载微藻油的电喷雾纳米颗粒对 PC-3、SHSY-5Y 和 AGS 细胞系的抗癌作用的比较。

制药工业中使用的许多生物活性物质易受温度、光照和氧气的影响，在储存和加工过程中容易降解，在消化过程中吸附性能差，限制了它们的直接使用。微藻富含具有抗菌特性和抗氧化剂的油，近年来在食品和制药领域引起了人们的关注。用纳米技术方法封装富含生物活性化合物的微藻油以提高物理和化学稳定性的研究相对较新，并且有望将这些方法应用于制药目的。在本研究中，布氏葡萄球菌和铜绿微囊藻的油提取物及其载油纳米颗粒对 L929 细胞系、PC-3 前列腺细胞系、研究了SHSY-5Y神经母细胞瘤细胞系和AGS胃腺癌细胞系。发现获得的提取物对L929细胞没有细胞毒性作用。然而，它们对癌细胞显示出细胞毒性作用。至于纳米粒子；确定了逐渐释放并显示了纳米颗粒结构的稳定性。根据获得的研究结果，认为用具有生物活性物质的微藻油提取物生产的纳米颗粒具有评估潜力，尤其是在制药和化妆品领域。确定了逐渐释放并显示了纳米颗粒结构的稳定性。根据获得的研究结果，认为用具有生物活性物质的微藻油提取物生产的纳米颗粒具有评估潜力，尤其是在制药和化妆品领域。确定了逐渐释放并显示了纳米颗粒结构的稳定性。根据获得的研究结果，认为用具有生物活性物质的微藻油提取物生产的纳米颗粒具有评估潜力，尤其是在制药和化妆品领域。