Membrane proteins are crucial for biological processes, and many of them are important to drug targets. Understanding the three-dimensional structures of membrane proteins are essential to evaluate their bio-function and drug design. High-purity membrane proteins are important for structural determination. Membrane proteins have low yields and are difficult to purify because they tend to aggregate. We summarized membrane protein expression systems, vectors, tags, and detergents, which have deposited in the Protein Data Bank (PDB) in recent four-and-a-half years. Escherichia coli is the most expression system for membrane proteins, and HEK293 cells are the most commonly cell lines for human membrane protein expression. The most frequently vectors are pFastBac1 for alpha-helical membrane proteins, pET28a for beta-barrel membrane proteins, and pTRC99a for monotopic membrane proteins. The most used tag for membrane proteins is the 6×His-tag. FLAG commonly used for alpha-helical membrane proteins, Strep and GST for beta- barrel and monotopic membrane proteins, respectively. The detergents and their concentrations used for alpha-helical, beta-barrel, and monotopic membrane proteins are different, and DDM is commonly used for membrane protein purification. It can guide the expression and purification of membrane proteins, thus contributing to their structure and bio function studying.

膜蛋白对生物过程至关重要，其中许多对药物靶标很重要。了解膜蛋白的三维结构对于评估其生物功能和药物设计至关重要。高纯度膜蛋白对于结构测定很重要。膜蛋白产量低，难以纯化，因为它们容易聚集。我们总结了最近四年半存放在蛋白质数据库 (PDB) 中的膜蛋白表达系统、载体、标签和去污剂。大肠杆菌是膜蛋白表达最多的系统，HEK293细胞是人膜蛋白表达最常用的细胞系。最常见的载体是 pFastBac1 用于 α 螺旋膜蛋白，pET28a 用于 β 桶膜蛋白，和 pTRC99a 用于单位膜蛋白。膜蛋白最常用的标签是 6×His 标签。FLAG 通常用于 α-螺旋膜蛋白，Strep 和 GST 分别用于β-桶状膜蛋白和单质膜蛋白。用于α-螺旋、β-桶和单一膜蛋白的去污剂及其浓度不同，DDM 常用于膜蛋白纯化。它可以指导膜蛋白的表达和纯化，从而有助于其结构和生物功能的研究。与单质膜蛋白不同，DDM 常用于膜蛋白纯化。它可以指导膜蛋白的表达和纯化，从而有助于其结构和生物功能的研究。与单质膜蛋白不同，DDM 常用于膜蛋白纯化。它可以指导膜蛋白的表达和纯化，从而有助于其结构和生物功能的研究。