Abstract

Dormancy release and reactivation of temperate-zone trees involve the temperature-modulated expression of cell-cycle genes. However, information on the detailed regulatory mechanism is limited. Here, we compared the transcriptomes of the stems of active and dormant larch trees, emphasizing the expression patterns of cell-cycle genes and transcription factors and assessed their relationships and responses to temperatures. Twelve cell-cycle genes and 31 transcription factors were strongly expressed in the active stage. Promoter analysis suggested that these 12 genes might be regulated by transcription factors from 10 families. Altogether, 73 cases of regulation between 16 transcription factors and 12 cell-cycle genes were predicted, while the regulatory interactions between LaMYB20 and LaCYCB1;1, and LaRAV1 and LaCDKB1;3 were confirmed by yeast one-hybrid and dual-luciferase assays. Last, we found that LaRAV1 and LaCDKB1;3 had almost the same expression patterns during dormancy release and reactivation induced naturally or artificially by temperature, indicating that the LaRAV1-LaCDKB1;3 module functions in the temperature-modulated dormancy release and reactivation of larch trees. These results provide new insights into the link between temperature and cell-cycle gene expression, helping to understand the temperature control of tree growth and development in the context of climate change.

中文翻译：

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落叶松休眠释放和重新激活期间温度对 LaRAV1-LaCDKB1;3 模块的协同控制

摘要

温带树木的休眠释放和重新激活涉及细胞周期基因的温度调节表达。然而，有关详细监管机制的信息有限。在这里，我们比较了活跃和休眠落叶松树干的转录组，强调了细胞周期基因和转录因子的表达模式，并评估了它们的关系和对温度的响应。12个细胞周期基因和31个转录因子在活跃期强烈表达。启动子分析表明这12个基因可能受到来自10个家族的转录因子的调控。总共预测了 16 个转录因子和 12 个细胞周期基因之间的 73 例调节，同时通过酵母单杂交和双荧光素酶测定证实了LaMYB20和LaCYCB1;1以及LaRAV1和LaCDKB1;3之间的调节相互作用。最后，我们发现LaRAV1和LaCDKB1;3在自然或人工温度诱导的休眠释放和重新激活过程中具有几乎相同的表达模式，表明LaRAV1 - LaCDKB1 ;3模块在落叶松温度调节的休眠释放和重新激活中发挥作用。这些结果为温度与细胞周期基因表达之间的联系提供了新的见解，有助于了解气候变化背景下树木生长和发育的温度控制。