BACKGROUND There is growing evidence that polymorphisms in chemokine and chemokine receptor genes influence susceptibility to HIV infection and disease progression. However, not much is documented about the influence of these polymorphisms in HIV serodiscordant couples in Cameroon. OBJECTIVE The objective of this study therefore was to determine the prevalence and the effect of the polymorphisms of CCR5-Δ32, CCR5 promoter 59029 A/G, CCR2-64I and SDF1-3'A gene in HIV serodiscordant couples in comparison to HIV negative seroconcordant and HIV positive seroconcordant couples in Yaoundé-Cameroon. METHOD A total of 96 couples were recruited from five hospitals, of which 60 couples were HIV serodiscordant (test group), 18 HIV negative seroconcordant and 18 HIV positive seroconcordant couples were used as controls. Their genotypes for CCR5-Δ32, CCR5 promoter, CCR2and SDF1-were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism. RESULTS The allelic frequencies of these genes in the studied population were: 0%, 26.30%, 15.30% and 1.62% respectively for CCR5-Δ32, CCR5 promoter, CCR2and SDF1. The frequency of the combination of CCR5 promoter and SDF1- (A/A+ G/G) wild-type genotype was higher in HIV-infected partners (82.92%) compared to uninfected partners (56.1%) in HIV serodiscordant couples (p= 0.0001). The combination of wild-type CCR2and SDF1genotypes (G/G + G/G) was higher among uninfected partners (80.48%) in HIV serodiscordant couples compared to the infected partners (60.97) (p= 0.005). CONCLUSION HIV negative partner protection against HIV/AIDS infection may be attributed to the combination of wild-typegenotypes (G/G and G/G) of CCR2and SDF1genes in HIV serodiscordant couples.

中文翻译：

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在雅温得喀麦隆，血清型CCR2-64V和SDF1-3A'基因的野生型基因型（G / G和G / G）的组合对保护性保护作用。

背景技术越来越多的证据表明趋化因子和趋化因子受体基因的多态性影响对HIV感染和疾病进展的敏感性。但是，关于喀麦隆的艾滋病毒血清对夫妇中这些多态性的影响的文献报道很少。目的因此，本研究的目的是确定与血清学阴性的艾滋病毒血清相容性伴侣相比，血清学检测的夫妻中CCR5-Δ32，CCR5启动子59029 A / G，CCR2-64I和SDF1-3'A基因多态性的患病率及其影响和喀麦隆雅温得的HIV阳性血清伴侣。方法从五家医院共招募96对夫妇，其中60对夫妇为HIV血清溶血药（测试组），以18例HIV阴性血清顺应剂和18例HIV阳性血清顺应剂为对照。它们的CCR5-Δ32基因型 使用聚合酶链反应（PCR）和限制性片段长度多态性分析CCR5启动子，CCR2和SDF1。结果这些基因在CCR5-Δ32，CCR5启动子，CCR2和SDF1中的等位基因频率分别为：0％，26.30％，15.30％和1.62％。HIV感染伴侣中CCR5启动子和SDF1-（A / A + G / G）野生型基因型组合的频率更高（82.92％），而未感染伴侣（56.1％）则更高（p = 0.0001 ）。HIV血清抗体夫妇中未感染伴侣中野生型CCR2和SDF1基因型（G / G + G / G）的组合要高于感染伴侣（60.97）（p = 0.005）（80.48％）。