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Palmitic acid decreases cell migration by increasing RGS2 expression and decreasing SERCA expression.
Genetics and Molecular Biology ( IF 2.1 ) Pub Date : 2021-03-15 , DOI: 10.1590/1678-4685-gmb-2020-0279
Octavio Galindo-Hernandez,Ana Gabriela Leija-Montoya,Tatiana Romero-Garcia,Jose Gustavo Vazquez-Jimenez

Palmitic acid, the main saturated fatty acid, is related with a wide range of metabolic disorders such as obesity, type 2 diabetes and heart disease. It is known that palmitic acid disturbs the expression of some important proteins for cell homeostasis such as SERCA and RGS2, however, the role of this lipid at the molecular level in these disorders is not completely elucidated. Thus, our aim was to determinate the effect of palmitic acid in a relevant cell process as it is cell migration and the participation of SERCA and RGS2 in this response. We found that palmitic acid reduces cell migration (determined by the Boyden chamber method) in an epithelial cell line (HEK293) and this effect is modulated by SERCA and RGS2 differential protein expression (measured by western blot). Also, overexpression of individual proteins, RGS2 and SERCA, produced a decrease and an increase on cell migration, respectively. Taken together, these data suggest that the expression of regulatory proteins is affected by high concentrations of saturated fatty acids and in consequence cell migration is diminished in epithelial cells.

中文翻译:

棕榈酸通过增加RGS2表达和减少SERCA表达来减少细胞迁移。

棕榈酸是主要的饱和脂肪酸,它与多种代谢异常有关,例如肥胖,2型糖尿病和心脏病。众所周知,棕榈酸会干扰一些用于细胞稳态的重要蛋白质的表达,例如SERCA和RGS2,但是,尚未完全阐明这种脂质在分子水平上在这些疾病中的作用。因此,我们的目标是确定棕榈酸在相关细胞过程中的作用,因为它是细胞迁移以及SERCA和RGS2参与此反应的过程。我们发现棕榈酸可减少上皮细胞系(HEK293)中的细胞迁移(由Boyden室法确定),并且这种作用受SERCA和RGS2差异蛋白表达(通过蛋白质印迹法测量)的调节。此外,个别蛋白质RGS2和SERCA的过度表达,分别导致细胞迁移减少和增加。综上所述,这些数据表明调节蛋白的表达受高浓度饱和脂肪酸的影响,因此上皮细胞中的细胞迁移减少。
更新日期:2021-03-15
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