Many cellular processes are orchestrated by dynamic changes in the plasma membrane to form membrane projections and endocytic vesicles in response to extracellular environmental changes. Our previous studies show that ARF6-ACAP4-ezrin signaling regulates membrane dynamics and curvature in response to EGF stimulation. However, there is no quantitative measurement to relate molecular organization of membrane cytoskeletal remodeling to stimulus-elicited mechanosensation on the plasma membrane. Optical tweezers is a powerful tool in the study of membrane tension. Comparing to pulling out an entire membrane tether at one time, the step-like method is more efficient because multiple relaxation curves can be obtained from one membrane tether. Fewer models describe relaxation curves to characterize mechanical properties of cell membrane. Here we establish a new method to measure the membrane relaxation curve of HeLa cells judged by the relationship between membrane tether diameter and tensions. We obtained effective viscosities and static tensions by fitting relaxation curves to our model. We noticed the delicate structure of relaxation curves contains information of cytoskeletal remodeling and lateral protein diffusion. Our study established a quantitative measure to characterize the mechanosensation of epithelial cells in response to stimulus-elicited membrane dynamics.

中文翻译：

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使用光学镊子测量动态膜机械感觉。

响应于细胞外环境变化，质膜的动态变化会协调许多细胞过程，从而形成膜突起和内吞小泡。我们以前的研究表明，ARF6-ACAP4-ezrin信号调节EGF刺激响应的膜动力学和曲率。然而，尚无定量测量将膜细胞骨架重塑的分子组织与质膜上的刺激引起的机械感觉相关。镊子是研究膜张力的有力工具。与一次拉出整个膜系绳相比，阶梯状方法更有效，因为可以从一个膜系绳获得多个松弛曲线。较少的模型描述弛豫曲线来表征细胞膜的机械性能。在这里，我们建立了一种新的方法来测量HeLa细胞的膜松弛曲线，该曲线是通过膜系绳直径和张力之间的关系判断的。通过将松弛曲线拟合到模型中，我们获得了有效的粘度和静态张力。我们注意到松弛曲线的微妙结构包含细胞骨架重塑和蛋白质横向扩散的信息。我们的研究建立了一种定量措施来表征上皮细胞对刺激引起的膜动力学的响应。我们注意到松弛曲线的微妙结构包含细胞骨架重塑和蛋白质横向扩散的信息。我们的研究建立了一种定量措施来表征上皮细胞对刺激引起的膜动力学的响应。我们注意到松弛曲线的微妙结构包含细胞骨架重塑和蛋白质横向扩散的信息。我们的研究建立了一种定量措施来表征上皮细胞对刺激引起的膜动力学的响应。