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Detection of 30 Fentanyl Analogs by Commercial Immunoassay Kits.
Journal of Analytical Toxicology ( IF 2.5 ) Pub Date : 2021-02-13 , DOI: 10.1093/jat/bkaa181
Rebekah E Wharton 1 , Jerry Casbohm 2 , Ryan Hoffmaster 2 , Bobby N Brewer 2 , M G Finn 3 , Rudolph C Johnson 1
Affiliation  

Health-care workers, laboratorians and overdose prevention centers rely on commercial immunoassays to detect the presence of fentanyl; however, the cross-reactivity of fentanyl analogs with these kits is largely unknown. To address this, we conducted a pilot study evaluating the detection of 30 fentanyl analogs and metabolites by 19 commercially available kits (9 lateral flow assays, 7 heterogeneous immunoassays and 3 homogenous immunoassays). The analogs selected for analysis were compiled from the Drug Enforcement Administration and National Forensic Laboratory Information System reports from 2015 to 2018. In general, the immunoassays tested were able to detect their intended fentanyl analog and some closely related analogs, but more structurally diverse analogs, including 4-methoxy-butyryl fentanyl and 3-methylfentanyl, were not well detected. Carfentanil was only detected by kits specifically designed for its recognition. In general, analogs with group additions to the piperidine, or bulky rings or long alkyl chain modifications in the N-aryl or alkyl amide regions, were poorly detected compared to other types of modifications. This preliminary information is useful for screening diagnostic, forensic and unknown powder samples for the presence of fentanyl analogs and guiding future testing improvements.

中文翻译:

通过商业免疫分析试剂盒检测30种芬太尼类似物。

卫生保健工作者,实验室工作人员和药物过量预防中心依靠商业免疫测定法来检测芬太尼的存在。但是,芬太尼类似物与这些试剂盒的交叉反应性在很大程度上尚不清楚。为了解决这个问题,我们进行了一项初步研究,评估了通过19种市售试剂盒(9种侧向血流测定,7种异质免疫测定和3种同质免疫测定)对30种芬太尼类似物和代谢产物的检测。选定用于分析的类似物摘自2015年至2018年美国药物管制局和国家法医实验室信息系统的报告。总体而言,所测试的免疫分析能够检测其预期的芬太尼类似物和一些密切相关的类似物,但结构上更多样化,包括4-甲氧基丁酰基芬太尼和3-甲基芬太尼在内的化合物未得到很好的检测。芬太尼仅通过专门为其识别而设计的试剂盒进行检测。通常,与其他类型的修饰相比,很难检测到在哌啶中具有基团加成的基团,或在N-芳基或烷基酰胺区域中存在大环或长烷基链修饰的类似物。此初步信息可用于筛选诊断性,法医和未知的粉末样品中是否存在芬太尼类似物,并指导将来的测试改进。
更新日期:2021-02-13
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