Basing on alternative splicing events (ASEs) databases, the authors herein aim to explore potential prognostic biomarkers for cervical squamous cell carcinoma (CESC). mRNA expression profiles and relevant clinical data of 223 patients with CESC were obtained from The Cancer Genome Atlas (TCGA). Correlated genes, ASEs and percent-splice-in (PSI) were downloaded from SpliceSeq, respectively. The PSI values of survival-associated alternative splicing events (SASEs) were used to construct the basis of a prognostic index (PI). A protein-protein interaction (PPI) network of genes related to SASEs was generated by STRING and analysed with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Consequently, 41,776 ASEs were discovered in 19,724 genes, 2596 of which linked with 3669 SASEs. The PPI network of SASEs related genes revealed that TP53 and UBA52 were core genes. The low-risk group had a longer survival period than high-risk counterparts, both groups being defined according to PI constructed upon the top 20 splicing events or PI on the overall splicing events. The AUC value of ROC reached up to 0.88, demonstrating the prognostic potential of PI in CESC. These findings suggested that ASEs involve in the pathogenesis of CESC and may serve as promising prognostic biomarkers for this female malignancy.

中文翻译：

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宫颈鳞状细胞癌的预后选择性剪接特征。

基于可变剪接事件 (ASEs) 数据库，本文作者旨在探索宫颈鳞状细胞癌 (CESC) 的潜在预后生物标志物。223 名 CESC 患者的 mRNA 表达谱和相关临床数据来自癌症基因组图谱 (TCGA)。相关基因、ASE 和剪接百分比 (PSI) 分别从 SpliceSeq 下载。生存相关可变剪接事件 (SASE) 的 PSI 值用于构建预后指数 (PI) 的基础。通过 STRING 生成与 SASE 相关的基因的蛋白质-蛋白质相互作用 (PPI) 网络，并使用基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 进行分析。因此，在 19,724 个基因中发现了 41,776 个 ASE，其中 2596 个与 3669 个 SASE 相关联。SASEs相关基因的PPI网络显示TP53和UBA52是核心基因。低风险组的生存期比高风险组长，两组均根据前 20 个剪接事件构建的 PI 或整体剪接事件的 PI 定义。ROC的AUC值达到0.88，证明了PI在CESC中的预后潜力。这些发现表明，ASEs 参与了 CESC 的发病机制，并可能作为这种女性恶性肿瘤的有希望的预后生物标志物。