To obtain native strains to efficiently use puerariae slag (PS) hydrolysate and resistant butanol toxicity, a self-designed isolation method was adopted. With this effort, the strain YBS3 was obtained. Based on 16S rDNA comparison, the strain YBS3 was identified as Clostridium beijerinckii. The feasibility of using PS hydrolysate as substrate to produce butanol was evaluated. A significant phase shift delay was encountered in acetone-butanol-ethanol (ABE) fermentation process, and then leaded to low production and productivity of butanol. However, when the C/N ratio regulation strategy was performed at mid-logarithmic phase, the problem of the phase shift delay was smoothly solved and the yield and productivity of butanol was improved. Specifically, the final production and productivity of butanol were 9.75 ± 0.14 g/L and 0.18 g/(L·h), respectively, which was 46.40% and 125.00% higher than without C/N ratio regulation. This study confirms that the accelerating phase shift is a convenient but effective approach, thereby is a promising technology for improving ABE fermentation performance.

为了获得有效利用葛根渣水解物和抗丁醇毒性的天然菌株，采用了自行设计的分离方法。通过这种努力，获得了菌株YBS3。基于16S rDNA比较，菌株YBS3被鉴定为拜氏梭菌。评估了使用PS水解产物作为底物生产丁醇的可行性。在丙酮-丁醇-乙醇（ABE）发酵过程中遇到了明显的相移延迟，然后导致丁醇的生产和生产率较低。然而，当在对数中期进行C / N比调节策略时，相移延迟的问题得到了解决，丁醇的收率和生产率得到了提高。具体而言，丁醇的最终产量和生产率分别为9.75±0.14 g / L和0.18 g /（L·h），比不使用C / N比例调节时分别高46.40％和125.00％。这项研究证实，加速相移是一种方便但有效的方法，因此是提高ABE发酵性能的有前途的技术。