Background: Emerging research has demonstrated that long non-coding RNAs (lncRNAs) attach great importance to the progression of cervical cancer (CC). LncRNA ARAP1-AS1 was involved in the development of several cancers; however, its role in CC is far from being elucidated. Methods: Real-time PCR (RT-PCR) was employed to detect ARAP1-AS1 and miR-149-3p expression in CC samples. CC cell lines (HeLa and C33A cells) were regarded as the cell models. The biological effect of ARAP1-AS1 on cancer cells was measured using CCK-8 assay, colony formation assay, flow cytometry, Transwell assay and wound healing assay in vitro, and subcutaneous xenotransplanted tumor model and tail vein injection model in vivo. Furthermore, interactions between ARAP1-AS1 and miR-149-3p, miR-149-3p and POU class 2 homeobox 2 (POU2F2) were determined by bioinformatics analysis, qRT-PCR, Western blot, luciferase reporter and RNA immunoprecipitation assay, respectively. Results: The expression of ARAP1-AS1 was enhanced in CC samples, while miR-149-3p was markedly suppressed. Additionally, ARAP1-AS1 overexpression enhanced the viability, migration, and invasion of CC cells. ARAP1-AS1 downregulated miR-149-3p via sponging it. ARAP1-AS1 and miR-149-3p exhibited a negative correlation in CC samples. On the other hand, ARAP1-AS1 enhanced the expression of POU2F2, which was validated as a target gene of miR-149-3p. Conclusion: ARAP1-AS1 was abnormally upregulated in CC tissues and indirectly modulated the POU2F2 expression via reducing miR-149-3p expression. Our study identified a novel axis, ARAP1-AS1/miR-149-3p/POU2F2, in CC tumorigenesis.
Pathobiology

中文翻译：

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长链非编码 RNA ARAP1-AS1 通过调节 miR-149-3p 和 POU2F2 促进宫颈癌的进展

背景：新兴研究表明，长链非编码 RNA (lncRNA) 非常重视宫颈癌 (CC) 的进展。LncRNA ARAP1-AS1 参与了多种癌症的发展；然而，它在 CC 中的作用远未阐明。方法：采用实时 PCR (RT-PCR) 检测 CC 样品中的 ARAP1-AS1 和 miR-149-3p 表达。CC细胞系（HeLa和C33A细胞）被视为细胞模型。ARAP1-AS1对癌细胞的生物学效应通过体外CCK-8测定、集落形成测定、流式细胞术、Transwell测定和伤口愈合测定以及体内皮下异种移植肿瘤模型和尾静脉注射模型测定。此外，ARAP1-AS1 与 miR-149-3p、miR-149-3p 和 POU 2 类同源框 2 (POU2F2) 之间的相互作用分别通过生物信息学分析、qRT-PCR、蛋白质印迹、荧光素酶报告基因和 RNA 免疫沉淀测定确定。结果：CC 样品中 ARAP1-AS1 的表达增强，而 miR-149-3p 显着抑制。此外，ARAP1-AS1 过表达增强了 CC 细胞的活力、迁移和侵袭。ARAP1-AS1 通过海绵作用下调 miR-149-3p。ARAP1-AS1 和 miR-149-3p 在 CC 样本中表现出负相关。另一方面，ARAP1-AS1 增强了 POU2F2 的表达，POU2F2 被证实为 miR-149-3p 的靶基因。结论： ARAP1-AS1在CC组织中异常上调，并通过降低miR-149-3p的表达间接调节POU2F2的表达。我们的研究确定了一个新的轴，ARAP1-AS1/miR-149-3p/POU2F2，在 CC 肿瘤发生中。
病理生物学