Replacement of fish meal with high plant protein meal leads to reduced growth performance and health issues in farmed fish. Supplementation of functional additives has been proposed as a potential strategy to alleviate the negative effect of plant protein replacement. In this study, the effects of phosphatidic acid (PA) on growth and antioxidant capacity in juvenile turbot fed with high plant protein-based diets were evaluated. An 11-week feeding trial was performed using diets containing 0 (CON group), 0.05, 0.1, or 0.5% PA. The results showed that adding 0.5% PA could significantly improve the growth performance of turbot without affecting feed intake. Moreover, supplementation of 0.5% PA significantly increased the mRNA level of igf-1 in liver. The condition factor was also enhanced in PA addition groups. The activity of lipase was increased by PA supplementation, while no difference was observed in amylase and trypsin activity. Supplying PA significantly reduced the content of malondialdehyde. Furthermore, the mRNA levels of superoxide dismutase (sod), glutathione peroxidase (gpx), and peroxiredoxin 6 (prx6) were enhanced in 0.1 and 0.5% PA groups. Our results showed that PA might be a functional additive for aquafeed with growth-promoting and antioxidative effects.

中文翻译：

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磷脂酸对高植物蛋白饲料喂养的大菱鲆幼鱼生长和抗氧化能力的影响

用高植物蛋白粉代替鱼粉会导致养殖鱼类的生长性能下降和健康问题。已经提出补充功能性添加剂作为减轻植物蛋白替代的负面影响的潜在策略。在这项研究中，评估了磷脂酸 (PA) 对高植物蛋白饲料喂养的比目鱼幼鱼生长和抗氧化能力的影响。使用含有 0（CON 组）、0.05、0.1 或 0.5% PA 的饮食进行了为期 11 周的喂养试验。结果表明，添加0.5%的PA可显着提高大菱鲆的生长性能，且不影响采食量。此外，补充 0.5% PA 显着增加了igf-1的 mRNA 水平在肝脏。PA添加组的条件因子也增强。脂肪酶的活性通过 PA 补充剂增加，而淀粉酶和胰蛋白酶活性没有观察到差异。供应PA显着降低了丙二醛的含量。此外，超氧化物歧化酶( sod )、谷胱甘肽过氧化物酶( gpx ) 和过氧还蛋白6 ( prx6 )的 mRNA 水平在 0.1% 和 0.5% PA 组中增强。我们的研究结果表明，PA 可能是一种具有促进生长和抗氧化作用的水产饲料的功能性添加剂。