Cultural heritage built from limestone is prone to deterioration by chemical weathering, a natural process, that is enhanced by pollution. There are many historic monuments built from calcareous rocks that suffer from deterioration, and thus there have been a number of approaches over the last few decades to consolidate these types of rocks and surfaces. Using natural biological processes by fostering the activity of calcite-producing bacteria, also referred to as biomineralization, is one strategy that has also been commercialized. The base of proving the effectiveness of any surface treatment is the observation of the surface at sequential stages before and after treatment, as well as after exposure to weathering. Due to the heterogeneity of natural materials and processes, our aim was to observe identical test areas at the micron scale throughout the observation period. In order to achieve this on a tungsten SEM, we employed a beam deceleration accessory that allowed low kV imaging on non-conductive surfaces at a sufficiently high image resolution with a modified sample holder accommodating drill cores of 25 mm diameter and up to 15 mm height. The presented method is capable of producing time-sequenced images on the same test area on natural rock surface samples without manipulation for imaging purposes. This offers interesting perspectives for effective documentation of such processes in various fields.

用石灰石建造的文化遗产很容易因化学风化而恶化，化学风化是一种自然过程，而污染会加剧这种情况。有许多用石灰岩建造的历史古迹，这些古迹遭受了破坏，因此在过去的几十年中，已经采取了许多方法来巩固这些类型的岩石和地表。通过促进产生方解石的细菌的活动来利用自然生物学过程，也称为生物矿化，是一种已经商业化的策略。证明任何表面处理有效性的基础是在处理之前和之后以及暴露于风化之后的连续阶段观察表面。由于天然材料和工艺的异质性，我们的目标是在整个观察期间以微米级观察相同的测试区域。为了在钨SEM上实现这一目标，我们使用了光束减速附件，该附件允许在非导电表面上以足够高的图像分辨率进行低kV成像，并带有改进的样品架，可容纳直径为25毫米，高度最大为15毫米的钻芯。所提出的方法能够在天然岩石表面样本的相同测试区域上产生按时间顺序排列的图像，而无需进行成像操作。这为有效记录各个领域的过程提供了有趣的观点。我们使用了光束减速附件，该光束减速附件可以在非导电表面上以足够高的图像分辨率进行低kV成像，并使用可容纳25毫米直径和最大15毫米高度的改进型样品架。所提出的方法能够在天然岩石表面样本的相同测试区域上产生按时间顺序排列的图像，而无需进行成像操作。这为有效记录各个领域的过程提供了有趣的观点。我们使用了光束减速附件，该光束减速附件可以在非导电表面上以足够高的图像分辨率进行低kV成像，并使用可容纳25毫米直径和最大15毫米高度的改进型样品架。所提出的方法能够在天然岩石表面样本的相同测试区域上产生按时间顺序排列的图像，而无需进行成像操作。这为有效记录各个领域的过程提供了有趣的观点。