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An “on-off” SERS Aptasensor Based on DNAzyme-Gold Nanorod for Ultrasensitive Pb2+ Detection in Human Serum
Analytica Chimica Acta: X Pub Date : 2019-07-01 , DOI: 10.1016/j.acax.2019.100020
Wei Xu 1, 2, 3 , Aiwu Zhao 1, 2, 3 , Fangtao Zuo 1, 2, 3 , Hafiz Muhammad Jafar Hussain 4 , Ranjha Khan 4
Affiliation  

It is great significance to precisely monitor lead (II) ions (Pb2+) for environment protection and human health monitoring. We designed a sensitive detection strategy for sensitive and selective determination of Pb2+, based on a Pb2+-specific DNAzyme as the catalytic unit, Cy3-labeled DNA modified gold nanorods (AuNRs) as SERS reporter. Firstly, AuNRs surface were employed as a platform for the immobilization of thiolated probe DNA, and then hybridized with DNAzyme catalytic beacons. By taking advantage of DNAzyme digest, a molecular beacon, causes a “turn-off” SERS signal by disrupting the labeled probes. Under the optical conditions, the DNAzyme-AuNRs sensor system exhibited high sensitivity, acceptable stability and reproducibility with a wide linear range from 0.5 to 100 nM (R2 = 0.9973), and an ultra-low detection limit of 0.01 nM. The proposed strategy has additional advantages of being less time-consuming, low-cost and remote query, and avoids the interference of some metals such as Fe3+, Cd2+, Ba2+, Cu2+, Zn2+. The SERS biosensor system has been successfully applied for detecting Pb2+ in real samples with a satisfactory result. The result indicated that the proposed sensing strategy not only enriches SERS platform of monitoring Pb2+ but also exhibits potential for the point-of-care diagnostic application of the clinical screening in complicated biological samples.

中文翻译:

基于 DNAzyme-Gold Nanorod 的“开关”SERS 适体传感器用于超灵敏检测人血清中的 Pb2+

精确监测铅(II)离子(Pb2+)对于环境保护和人体健康监测具有重要意义。我们设计了一种灵敏检测策略,以 Pb2+ 特异性 DNAzyme 作为催化单元,Cy3 标记的 DNA 修饰金纳米棒 (AuNRs) 作为 SERS 报告基因,用于灵敏和选择性测定 Pb2+。首先,AuNRs 表面被用作固定硫醇化探针 DNA 的平台,然后与 DNAzyme 催化信标杂交。通过利用 DNAzyme 消化,分子信标通过破坏标记的探针导致“关闭”SERS 信号。在光学条件下,DNAzyme-AuNRs 传感器系统表现出高灵敏度、可接受的稳定性和重现性,线性范围从 0.5 到 100 nM(R2 = 0.9973),超低检测限为 0.01 nM。所提出的策略具有耗时少、成本低和远程查询的额外优势,并避免了一些金属如 Fe3+、Cd2+、Ba2+、Cu2+、Zn2+ 的干扰。SERS生物传感器系统已成功应用于实际样品中Pb2+的检测,结果令人满意。结果表明,所提出的传感策略不仅丰富了监测 Pb2+ 的 SERS 平台,而且在复杂生物样本中临床筛查的即时诊断应用方面也具有潜力。
更新日期:2019-07-01
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