Chromosomal data are important for taxonomists, cytogeneticists and evolutionary biologists; however, the value of these data decreases sharply if they are obtained for individuals with inaccurate species identification or unclear species identity. To avoid this problem, here we suggest linking each karyotyped sample with its DNA barcode, photograph and precise geographic data, providing an opportunity for unambiguous identification of described taxa and for delimitation of undescribed species. Using this approach, we present new data on chromosome number diversity in neotropical butterflies of the subfamily Biblidinae (genus Vila Kirby, 1871) and the tribe Ithomiini (genera Oleria Hübner, 1816, Ithomia Hübner, 1816, Godyris Boisduval, 1870, Hypothyris Hübner, 1821, Napeogenes Bates, 1862, Pseudoscada Godman et Salvin, 1879 and Hyposcada Godman et Salvin, 1879). Combining new and previously published data we show that the species complex Oleria onega (Hewitson, [1852]) includes three discrete chromosomal clusters (with haploid chromosome numbers n = 15, n = 22 and n = 30) and at least four DNA barcode clusters. Then we discuss how the incomplete connection between these chromosomal and molecular data (karyotypes and DNA barcodes were obtained for different sets of individuals) complicates the taxonomic interpretation of the discovered clusters.

中文翻译：

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将核型与DNA条码关联：基于对新热带tropic科（鳞翅目）的研究，提出了一个染色体分析新标准的实例。

染色体数据对于分类学家，细胞遗传学家和进化生物学家而言非常重要。但是，如果这些数据是针对物种识别不正确或物种身份不明确的个人获得的，则其价值将急剧下降。为避免此问题，在这里我们建议将每个核型样品与其DNA条码，照片和精确的地理数据联系起来，为明确地描述所描述的类群和界定未描述的物种提供机会。使用这种方法，我们介绍了Biblidinae亚科（维拉·柯比属，1871年）和Ithomiini部落（OleriaHübner属，1816年，IthomiaHübner，1816年，Godyris Boisduval，1870年，HypothyrisHübner，， 1821年，Napeogenes Bates，1862年，Pseudoscada Godman et Salvin，1879年以及Hyposcada Godman等人，Salvin，1879年）。结合新数据和先前发布的数据，我们发现物种复合物Oleria onega（Hewitson，[1852]）包括三个离散的染色体簇（单倍染色体编号n = 15，n = 22和n = 30）和至少四个DNA条码簇。然后，我们讨论了这些染色体和分子数据之间的不完全联系（对于不同的个体集获得了核型和DNA条形码）如何使发现的簇的分类学解释复杂化。