Abstract

Peripheral activities of kisspeptin (Kp) have been investigated in several studies and it was shown that Kp-10 activates ERK1/2, however, its regulation of AKT and mTOR activity is controversial. Therefore, this study aimed to investigate the effect of Kp-10 on cardiac ERK1/2 and AKT activities, as well as oxidative and antioxidative parameters in healthy mice. Twelve young C57/BL6 mice were divided into two groups with six animals in each group as follows: (1) Control that received only physiological saline and (2) Kp-10 that received 20 nM Kp-10 in physiological saline intraperitoneally. After a 3-h period, animals were sacrificed, and cardiac tissues were collected for the western blot and oxidative and antioxidative enzyme activities. AKT phosphorylation was significantly increased by Kp-10. In addition, Kp-10 significantly decreased mTOR activity. Superoxide dismutase, catalase, glutathione and malondialdehyde levels, as well as total oxidant status and oxidative stress index, were similar in both groups. Total antioxidant status was significantly higher in Kp-10 group. AKT is an upstream factor of the mTOR/S6 pathway, therefore, our results suggest that Kp-10 acts differentially on AKT and mTOR/S6 activities. Moreover, increased TAS suggests an antioxidant activity of Kp-10 in healthy heart.

中文翻译：

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Kisspeptin-10给药可调节小鼠心脏组织中的mTOR和AKT活性以及氧化应激。

摘要

已在几项研究中对kisepteptin（Kp）的外周活性进行了研究，结果表明Kp-10可以激活ERK1 / 2，但是其对AKT和mTOR活性的调控存在争议。因此，本研究旨在研究Kp-10对健康小鼠心脏ERK1 / 2和AKT活性以及氧化和抗氧化参数的影响。将十二只C57 / BL6幼鼠分成两组，每组六只动物，如下：（1）仅接受生理盐水的对照组和（2）腹膜内接受20 nM Kp-10的Kp-10。3小时后，处死动物，收集心脏组织进行Western印迹以及氧化和抗氧化酶活性。Kp-10使AKT磷酸化显着增加。此外，Kp-10显着降低mTOR活性。两组的超氧化物歧化酶，过氧化氢酶，谷胱甘肽和丙二醛水平以及总氧化剂状态和氧化应激指数相似。Kp-10组的总抗氧化剂状态显着更高。AKT是mTOR / S6途径的上游因素，因此，我们的结果表明Kp-10对AKT和mTOR / S6活性的作用不同。此外，TAS升高提示健康心脏中Kp-10的抗氧化活性。我们的结果表明，Kp-10对AKT和mTOR / S6活性的作用不同。此外，TAS升高提示健康心脏中Kp-10的抗氧化活性。我们的结果表明，Kp-10对AKT和mTOR / S6活性的作用不同。此外，TAS升高提示健康心脏中Kp-10的抗氧化活性。