Enterobacter cloacae (E. cloacae) is an emerging nosocomial pathogen that had acquired antibiotic resistance against multiple classes of antibiotics. The current study was aimed to isolate and characterize lytic bacteriophage against E. cloacae. The bacteriophage EBP was isolated from a sewage water sample using E. cloacae as a host strain by double-layer agar technique. EBP was found stabile at a wide range of temperatures (25, 37, 60, and 80°C) and pH (5, 6, 7, 8, and 9) with antibacterial activity up to 24 h of infection. The latent period of EBP was 20 min with a burst size of 252 phages per cell. It showed a narrow host range and infected 12/21 (57%) isolates of E. cloacae tested. It has helical symmetry with a head size of 105 and 120 nm long tail with contractile sheath. The EBP has 179.1 kb long double-stranded DNA genome with 44.8% GC content. Majority of identified ORFs (187/281) were encoding putative proteins with unknown function. Necessary replication enzymes, structural proteins, and lytic enzymes were detected in the genome of EBP. Phylogenetic analysis revealed that EBP closely resembles with Coronobacter phage vB_CsaM_IeN, vB_CsaM_IeE, vB_CsaM_IeB, and Citrobacter phage Margaery. Based on electron microscopy and molecular characterization, EBP was classified as a Myoviridae phage.

中文翻译：

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具有抗阴沟肠杆菌的大基因组裂解 EBP 噬菌体的表征

阴沟 肠杆菌（E. cloacae）是一种新兴的医院病原体，已对多种抗生素产生耐药性。目前的研究旨在孤立和打击表征裂解噬菌体ê。 泄殖腔。以阴沟肠杆菌为宿主菌株，通过双层琼脂技术从污水样品中分离出噬菌体EBP 。发现 EBP 在很宽的温度范围（25、37、60 和 80°C）和 pH 值（5、6、7、8 和 9）下都是稳定的，并且在感染后长达 24 小时具有抗菌活性。EBP 的潜伏期为 20 分钟，每个细胞的爆发大小为 252 个噬菌体。它显示出狭窄的宿主范围并感染了 12/21 (57%)阴沟肠杆菌分离株测试。它具有螺旋对称性，头部大小为 105 纳米，尾长为 120 纳米，具有可收缩的鞘。EBP 具有 179.1 kb 长的双链 DNA 基因组，GC 含量为 44.8%。大多数已鉴定的 ORF (187/281) 编码具有未知功能的假定蛋白质。在 EBP 的基因组中检测到必要的复制酶、结构蛋白和裂解酶。系统发育分析表明，EBP 与Coronobacter噬菌体 vB_CsaM_IeN、vB_CsaM_IeE、vB_CsaM_IeB 和柠檬酸杆菌噬菌体 Margaery 非常相似。基于电子显微镜和分子表征，EBP 被归类为肌病毒科噬菌体。