Microbial mineralization offers an efficient alternative to obtain metallic nanoparticles. The aim of this work was to synthesize green silver nanoparticles (AgNPs) using the metal resistant strain Streptomyces sp. M7. In addition, molecular modeling and docking studies were performed in order to understand how stabilizing proteins interact with the nanoparticle’s surface. The production of AgNPs was influenced by the incubation time. AgNPs showed a moderate polydispersity of size and spherical shape. The hydrodynamic diameter was determined by Dynamic Light Scattering (DLS). Fourier Transform Infrared Spectroscopy (FTIR) and Raman analysis showed that organic molecules such as oxide-reduction enzymes coated the AgNPs surface. Molecular modeling and docking studies indicated that actually different enzymes may be simultaneously bound to the nanoparticle surface forming a layer capping-protein with different affinities. Thermogravimetric analysis (TGA) showed high thermal resistance of AgNPs, probably by the presence of organic residues on the surface. The green synthesized AgNPs showed antimicrobial activity against several multidrug-resistant bacteria, particularly Serratia marcescens SmCR371. This strain belongs to a cluster with epidemic behavior, harboring an extended spectrum of $\beta$-lactamases and carbapenemase. AgNPs produced by Streptomyces sp. M7 could be considered as a new line of defense against microbial resistance or as a complement of traditional antibiotics.

微生物矿化为获得金属纳米颗粒提供了有效的替代方法。这项工作的目的是使用具有金属抗性的菌株Streptomyces合成绿色银纳米颗粒（AgNP）sp。M7。此外，进行了分子建模和对接研究，以了解稳定蛋白如何与纳米颗粒表面相互作用。AgNPs的产生受温育时间的影响。AgNPs表现出中等大小和球形的多分散性。流体动力学直径是通过动态光散射（DLS）确定的。傅立叶变换红外光谱（FTIR）和拉曼分析表明，有机分子（例如氧化还原酶）覆盖了AgNPs表面。分子建模和对接研究表明，实际上不同的酶可能同时与纳米颗粒表面结合，形成具有不同亲和力的覆盖蛋白层。热重分析（TGA）显示出AgNP的高耐热性，可能是由于表面上存在有机残留物所致。粘质沙雷氏菌SmCR371。该菌株属于具有流行行为的类群，具有扩展的$\beta$-内酰胺酶和碳青霉烯酶。链霉菌属物种产生的AgNP 。M7可以被视为对抗微生物耐药性的新防线，也可以被视为传统抗生素的补充。