The human enzyme As(III) S-adenosylmethionine methyltransferase (AS3MT) catalyzes arsenic biotransformations and is considered to contribute to arsenic-related diseases. AS3MT is expressed in various tissues and cell types including liver, brain, adrenal gland, and peripheral blood mononuclear cells but not in human keratinocytes, urothelial, or brain microvascular endothelial cells. This indicates that AS3MT expression is regulated in a tissue/cell type-specific manner, but the mechanism of transcriptional regulation of expression of the AS3MT gene is not known. In this study, we define the DNA sequence of the core promoter region of the human AS3MT gene. We identify a GC box in the promoter to which the stress-related transcription factor Sp1 binds, indicating involvement of regulatory elements in AS3MT gene expression.

Graphical abstract

中文翻译：

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砷甲基化的调控：人类 AS3MT 基因转录区域的鉴定

人类酶 As(III) S-腺苷甲硫氨酸甲基转移酶 (AS3MT) 催化砷生物转化，被认为与砷相关疾病有关。AS3MT 在多种组织和细胞类型中表达，包括肝、脑、肾上腺和外周血单核细胞，但在人角质形成细胞、尿路上皮或脑微血管内皮细胞中不表达。这表明AS3MT表达以组织/细胞类型特异性方式受到调节，但AS3MT基因表达的转录调节机制尚不清楚。在这项研究中，我们定义了人类 AS3MT 基因核心启动子区域的 DNA 序列。我们在启动子中发现了一个与应激相关转录因子 Sp1 结合的 GC 盒，表明调控元件参与了 AS3MT 基因表达。

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