Abstract

PDMS is the most widely used material in the microfluidic systems because of its advantageous properties including ease of manufacture, low cost, transparency, and gas permeability. Although all biomaterials need to be sterilized for usage for in vitro or in vivo systems to avoid possible contaminants, none of them proved effective in providing the gold standards so far for PDMS sterilization. Therefore we evaluated the influence of both UV and ethanol sterilization of PDMS substrates on the cell behavior via investigating cell proliferation, oxidative stress and nutrient metabolism of 293 T cells growing on the PDMS first time in the literature. Modulus of elasticity and stress relaxation of UV-treated PDMS samples increased compared to the ethanol-treated ones leading to a state of surface stiffness preferable for cell growth and proliferation. Oxidative stress metabolism of cells growing on the UV-treated PDMS was more stable compared to the ethanol-treated samples. Trace element and mineral metabolism are not impaired in the cells growing on the UV-treated PDMS samples. First time in the literature, we showed that UV treatment is more suitable for the PDMS samples used in the cell culture experiments.

摘要

PDMS 是微流体系统中使用最广泛的材料，因为它具有易于制造、成本低、透明性和透气性等优点。尽管所有生物材料都需要进行灭菌以用于体外或体内系统以避免可能的污染物，但迄今为止，它们都没有被证明能有效地为 PDMS 灭菌提供金标准。因此，我们通过研究文献中首次在 PDMS 上生长的 293 个 T 细胞的细胞增殖、氧化应激和营养代谢，评估了 PDMS 底物的紫外线和乙醇灭菌对细胞行为的影响。与乙醇处理的样品相比，UV 处理的 PDMS 样品的弹性模量和应力松弛增加，导致表面刚度状态更适合细胞生长和增殖。与乙醇处理的样品相比，在紫外线处理的 PDMS 上生长的细胞的氧化应激代谢更稳定。在紫外线处理的 PDMS 样品上生长的细胞中，微量元素和矿物质代谢不会受到损害。在文献中，我们首次表明紫外线处理更适合用于细胞培养实验的 PDMS 样品。