A method for the simultaneous determination of seven B-group vitamers including thiamine, riboflavin, nicotinamide, niacin, pyridoxine, pyridoxal, and pyridoxamine in nutritional products by using enzymatic digestion followed by LC-MS/MS quantification was studied. The LC-MS/MS conditions such as MS transitions, mobile phase programs, and ammonium formate buffer concentrations, and sample treatment procedures (e.g., concentrations of buffer solution, digestion temperature, and digestion time) were investigated. The analytical method performance was evaluated by multiple criteria such as selectivity, linearity, detection and quantification limits, repeatability, reproducibility, and recovery by using real sample matrices. The validated method was successfully applied to analyze vitamin B concentrations in different nutritional products like ultra-heat-treated milk, powdered milk, and nutritional powder. Vitamin B concentrations varied over a wide range from lower than detection limits to about 9000 µg/100 g, depending on vitamin groups, compound forms, and sample types. The measured concentrations of B-group vitamins in our samples were generally in good agreement with values of label claims.

中文翻译：

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营养产品中B组维生素的简单分析方法的开发：酶消化和UPLC-MS / MS定量

研究了一种同时测定营养产品中硫胺素，核黄素，烟酰胺，烟酸，吡ido醇，吡ido醛和吡x胺的7种B组维生素的方法，方法是酶促消化，然后进行LC-MS / MS定量分析。研究了LC-MS / MS条件，例如MS转换，流动相程序和甲酸铵缓冲液浓度，以及样品处理程序（例如缓冲液浓度，消化温度和消化时间）。分析方法的性能通过多种标准进行评估，例如选择性，线性，检测和定量限，可重复性，可重复性以及使用真实样品基质的回收率。经验证的方法已成功地用于分析不同营养产品（如超热处理牛奶，奶粉和营养粉）中的维生素B浓度。维生素B的浓度范围很广，从低于检出限到大约9000 µ g / 100 g，取决于维生素组，化合物形式和样品类型。我们样本中B组维生素的测量浓度通常与标签声明的值高度吻合。