Phosphodiesterase type 5 inhibitors (PDE-5i) are the first-line medication for oral erectile dysfunction, which are used according to the prescription of doctors. However, these substances have been found illegally in supplementary foods. The quality and safety of dietary supplements for enhancing male sexual performance have been questioned, raising the need for continual development of analytical methods. Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. In this research, we focused on (i) developing and validating an effective screening and quantitation method for more than 53 PDE-5i in ingredients and supplementary products using LC-Q-Exactive after a simple sample extraction and (ii) assessing PDE-5i content in natural-based supplementary products available in Vietnam market. The extraction method used a small amount of organic solvent, which makes it more environmentally friendly (greener). The developed method has a limit of detection of 0.4 mg/kg, a limit of quantitation of 1.2 mg/kg, recoveries from 80 to 110%, and repeatability lower than 15%. Ninety-two herbal supplementary foods and ingredients used for enhancement of male sexual performance available in Vietnamese markets were collected. Fourteen PDE-5i including conventional and novel analogous were detected and measured in eighteen food supplements and two formulation ingredient samples.

中文翻译：

---

膳食补充剂中非法磷酸二酯酶5型抑制剂（PDE-5i）的快速筛选和定量测定

磷酸二酯酶5型抑制剂（PDE-5i）是口服勃起功能障碍的一线药物，根据医生的处方使用。但是，在辅助食品中非法发现了这些物质。人们对提高男性性能力的膳食补充剂的质量和安全性提出了质疑，从而提出了不断发展分析方法的需求。液相色谱结合高分辨率质谱已成为鉴定和测量PDE-5i浓度的最有效方法之一。在这项研究中 我们着重于（i）通过简单的样品提取后，使用LC-Q-Exactive开发和验证一种有效的筛选和定量方法，用于成分和补充产品中的53种以上PDE-5i，以及（ii）评估天然-越南市场上提供的基于补充的产品。提取方法使用了少量的有机溶剂，这使其对环境更加友好（绿色）。所开发的方法的检出限为0.4 mg / kg，定量限为1.2 mg / kg，回收率从80％到110％，重复性低于15％。收集了九十二种在越南市场上可用于增强男性性能力的草药辅助食品和成分。