HNO (nitroxyl, IUPAC name azanone) is an electrophilic reactive nitrogen species of growing pharmacological and biological significance. Here, we present data on the pH-dependent kinetics of azanone reactions with the low molecular thiols glutathione and N-acetylcysteine, as well as with important serum proteins: bovine serum albumin and human serum albumin. The competition kinetics method used is based on two parallel HNO reactions: with RSH/RS⁻ or with O₂. The results provide evidence that the reaction of azanone with the anionic form of thiols (RS⁻) is favored over reactions with the protonated form (RSH). The data are supported with quantum mechanical calculations. A comprehensive discussion of the HNO reaction with thiolates is provided.

HNO（硝酰基，IUPAC 名称 azanone）是一种具有日益增长的药理学和生物学意义的亲电活性氮物种。在这里，我们展示了氮杂酮与低分子硫醇谷胱甘肽和N-乙酰半胱氨酸以及重要的血清蛋白（牛血清白蛋白和人血清白蛋白）反应的 pH 依赖性动力学数据。使用的竞争动力学方法基于两个平行的HNO反应：与RSH / RS ^-或与O ₂。结果提供了证据表明氮杂酮与阴离子形式的硫醇 ( RS ^- ) 的反应优于与质子化形式 ( RSH ) 的反应。）。数据得到量子力学计算的支持。提供了HNO与硫醇盐反应的全面讨论。