The barrier-to-autointegration factor (BAF) is widely expressed in most human tissues and plays a critical role in chromatin organization, nuclear envelope assembly, gonadal development, and embryonic stem cell self-renewal. Complete loss of BAF has been shown to lead to embryonic lethality and gonadal defects. The BAF paralog, namely, barrier-to-autointegration factor 2 (BANF2), exhibits a testis-predominant expression pattern in both humans and mice. Unlike BAF, it may cause isolated male infertility. Therefore, we used the CRISPR/Cas9 system to generate Banf2-knockout mice to further study its function in spermatogenesis. Unexpectedly, knockout mice did not show any detectable abnormalities in histological structure of the testis, epididymis, ovary, and other tissues, and exhibited normal fertility, indicating that Banf2 is not essential for mouse spermatogenesis and fertility.
Cytogenet Genome Res

中文翻译：

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Mus musculus Barrier-To-Autointegration Factor 2 (Banf2) 对精子发生或生育力不是必需的

自动整合屏障因子 (barrier-to-autointegration factor, BAF ) 在大多数人体组织中广泛表达，在染色质组织、核膜组装、性腺发育和胚胎干细胞自我更新中起着关键作用。BAF 的完全丧失已被证明会导致胚胎致死和性腺缺陷。的BAF旁系同源物，即，阻挡到自动积分因子2（BANF2），显示出在人类和小鼠中睾丸为主的表达模式。与BAF不同，它可能导致孤立的男性不育症。因此，我们使用 CRISPR/Cas9 系统生成Banf2-敲除小鼠以进一步研究其在精子发生中的功能。出乎意料的是，敲除小鼠的睾丸、附睾、卵巢和其他组织的组织学结构没有任何可检测到的异常，并且表现出正常的生育能力，表明Banf2对小鼠的精子发生和生育能力不是必需的。
细胞遗传基因组研究