Glucose dehydrogenase, as an important biocatalyst, has been widely used in biosensors, especially in measuring blood glucose levels. The low stability of the enzyme limits its applications in clinics and industry. To improve the stability, graphene oxide nanoparticles were fabricated, characterized, and used to immobilize of glucose dehydrogenase. The nanoparticles were characterized by FTIR. The enzyme was covalently immobilized on the nanoparticles with the activation of glutaraldehyde. After optimizing the immobilization conditions, an immobilization yield of 92% and the activity recovery of 91% were achieved. The immobilized GDH was characterized and compared with the free enzyme. It showed 6.9-fold higher stability than that of the free enzyme after the immobilization. And after 25 cycles, the immobilized GDH remained 50% of the initial activity. These results indicated that the immobilization could broaden the applications of the GDH in the industry and clinic.

葡萄糖脱氢酶作为一种重要的生物催化剂，已被广泛用于生物传感器，特别是在测量血糖水平方面。该酶的低稳定性限制了其在临床和工业中的应用。为了提高稳定性，制备了氧化石墨烯纳米颗粒，对其进行了表征，并用于固定化葡萄糖脱氢酶。纳米颗粒通过FTIR表征。通过戊二醛的活化，将酶共价固定在纳米颗粒上。优化固定化条件后，固定化产率为92％，活性回收率为91％。固定化的GDH进行了表征，并与游离酶进行了比较。固定后的稳定性比游离酶高6.9倍。然后经过25个循环，固定化的GDH仍保持初始活性的50％。这些结果表明，固定化可以扩大GDH在工业和临床中的应用。