Rotaviruses infect cells by binding to specific cell surface molecules including gangliosides, heat shock protein cognate protein 70 (Hsc70), and some integrins. The characterization of cell surface receptors defining viral tropism is crucial for inhibiting entry into the normal cells or the cancer cells. In the present work, several tumor cell-adapted rotavirus isolates were tested for their interaction with some heat shock proteins (HSPs) present in the U-937 cells, derived from a human pleural effusion (histiocytic lymphoma monocyte). This interaction was examined by virus overlay protein-binding (VOPB), immunochemistry, immuno-dot blot assays, and flow cytometry. The results indicated that the rotavirus isolates studied were able to infect U937 cells by interacting with Hsp90, Hsp70, Hsp60, Hsp40, Hsc70, protein disulfide isomerase (PDI), and integrin β3, which are implicated in cellular proliferation, differentiation, and cancer development. Interestingly, these cellular proteins were found to be associated in lipid microdomains (rafts), facilitating in this way eventual sequential interactions of the rotavirus particles with the cell surface receptors. The rotavirus tropism for U937 cells through the use of these cell surface proteins made this rotavirus isolates an attractive target for the development of oncolytic strategies in the context of alternative and complementary treatment of cancer.

轮状病毒通过与特定的细胞表面分子结合来感染细胞，这些分子包括神经节苷脂、热休克蛋白同源蛋白 70 (Hsc70) 和一些整合素。定义病毒向性的细胞表面受体的表征对于抑制进入正常细胞或癌细胞至关重要。在目前的工作中，测试了几种适应肿瘤细胞的轮状病毒分离株与来自人类胸腔积液（组织细胞淋巴瘤单核细胞）的 U-937 细胞中存在的一些热休克蛋白 (HSP) 的相互作用。通过病毒覆盖蛋白结合 (VOPB)、免疫化学、免疫斑点印迹分析和流式细胞术检查了这种相互作用。结果表明，所研究的轮状病毒分离株能够通过与 Hsp90、Hsp70、Hsp60、Hsp40、Hsc70、蛋白质二硫键异构酶 (PDI)、和整合素 β3，它们与细胞增殖、分化和癌症发展有关。有趣的是，发现这些细胞蛋白与脂质微区（筏）相关，以这种方式促进轮状病毒颗粒与细胞表面受体的最终顺序相互作用。通过使用这些细胞表面蛋白对 U937 细胞的轮状病毒嗜性使这种轮状病毒分离物成为在癌症替代和补充治疗背景下开发溶瘤策略的有吸引力的目标。以这种方式促进轮状病毒颗粒与细胞表面受体的最终顺序相互作用。通过使用这些细胞表面蛋白对 U937 细胞的轮状病毒嗜性使这种轮状病毒分离物成为在癌症替代和补充治疗背景下开发溶瘤策略的有吸引力的目标。以这种方式促进轮状病毒颗粒与细胞表面受体的最终顺序相互作用。通过使用这些细胞表面蛋白对 U937 细胞的轮状病毒嗜性使这种轮状病毒分离物成为在癌症替代和补充治疗背景下开发溶瘤策略的有吸引力的目标。