Abstract

Phytotoxicological evaluation of ZnO nanoparticles (NPs) is important to determine the possible effects on ecosystems. Although bulk Zn²⁺ ions can be used by plants as an essential micronutrient, it has many adverse effects on living organisms in nano-size. In this study, the growth, the development, and the stress effects of ZnO and Ni-doped (5%) ZnO NPs on wheat (Triticum aestivum – İkizce 96) were comparatively evaluated. In the growth and development experiments seed germination rate, root-shoot elongation, seedling vigor, plant height, and chlorophyll content in plants were determined, and the plant stress was evaluated depending on superoxide dismutase, catalase (CAT), and ascorbate peroxidase (APX) activities. It was observed that the height of the plants was decreased with increasing the NPs treatments. The seed germination and chlorophyll content were affected by neither the pure ZnO nor Ni-doped ZnO NPs. The effects of ZnO were more pronounced on superoxide dismutase while Ni:ZnO on CAT, and APX.

摘要

ZnO 纳米粒子 (NPs) 的植物毒理学评估对于确定对生态系统的可能影响很重要。虽然大量的Zn ²⁺离子可以被植物用作必需的微量营养素，但它对纳米尺寸的生物体有许多不利影响。在本研究中，ZnO 和 Ni 掺杂 (5%) ZnO NPs 对小麦 ( Triticum aestivum ) 的生长、发育和胁迫效应– İkizce 96) 进行了比较评估。在生长发育实验中，测定了种子发芽率、根茎伸长率、幼苗活力、株高和植物叶绿素含量，并根据超氧化物歧化酶、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX ） 活动。观察到植物的高度随着NPs处理的增加而降低。种子萌发和叶绿素含量不受纯 ZnO 和 Ni 掺杂 ZnO NPs 的影响。ZnO 对超氧化物歧化酶的影响更为明显，而 Ni:ZnO 对 CAT 和 APX 的影响更为明显。