Journal of Natural Medicines ( IF 3.3 ) Pub Date : 2021-05-04 , DOI: 10.1007/s11418-021-01518-w Chihiro Sugita 1 , Atsushi Yamashita 2 , Shigetoshi Tsutsumi 3 , Hisahiro Kai 1 , Tohru Sonoda 4 , Hiroki Yoshida 1 , Ryuichi Yamamoto 1 , Yujiro Asada 2 , Masahiko Kurokawa 1
Brazilian propolis (AF-08) is a dietary supplement containing a variety of flavonoids. It is used worldwide as a folk medicine. Flavonoids and a diet of fruits and vegetables containing them have been shown to reduce the risk of cardiovascular diseases (CVDs). Most of CVDs are caused by arterial thrombus formation. A thrombus is formed by the interaction between adhesion and aggregation of platelets to damaged blood vessels and blood coagulation consisting of extrisic and intrinsic pathways. Platelet aggregation and blood coagulation are closely linked to thrombosis. Therefore, we evaluated the effectiveness of AF-08 or its component flavonoids against thrombosis by examining their inhibition of platelet aggregation and blood coagulation. Human platelet-rich plasma was incubated with serial dilutions of AF-08 for 10 min to assess its inhibitory effect on platelet aggregation caused by collagen. The inhibitory effect of AF-08 on blood coagulation was evaluated by the prothrombin time (PT) and activated partial thromboplastin time (APTT), which reflect the coagulation function of extrinsic and intrinsic pathways, respectively. AF-08 significantly inhibited collagen-induced platelet aggregation but not PT and APTT, indicating that AF-08 inhibited platelet aggregation but not blood coagulation. Among three flavonoids contained in AF-08, apigenin and chrysin obviously inhibited platelet aggregation but the inhibitory effect of kaempferol was less effective. The three flavonoids did not affect PT and APTT. The inhibitory activity of AF-08 on human platelet aggregation without affecting blood coagulation was suggested to be partially due to apigenin and chrysin. AF-08 may be effective in suppressing platelet-based arterial thrombus formation and reducing the risk of CVDs.
中文翻译:
巴西蜂胶 (AF-08) 抑制胶原蛋白诱导的血小板聚集而不影响凝血
巴西蜂胶 (AF-08) 是一种含有多种黄酮类化合物的膳食补充剂。它在世界范围内用作民间药物。类黄酮和含有它们的水果和蔬菜的饮食已被证明可以降低心血管疾病 (CVD) 的风险。大多数心血管疾病是由动脉血栓形成引起的。血栓是由血小板粘附和聚集到受损血管与血液凝固之间的相互作用形成的,该凝血由外在和内在途径组成。血小板聚集和血液凝固与血栓形成密切相关。因此,我们通过检查 AF-08 或其成分类黄酮对血小板聚集和凝血的抑制作用来评估它们对血栓形成的有效性。将富含血小板的血浆与连续稀释的 AF-08 一起孵育 10 分钟,以评估其对胶原蛋白引起的血小板聚集的抑制作用。AF-08对凝血的抑制作用通过凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)来评价,它们分别反映外源性和内源性途径的凝血功能。AF-08 显着抑制胶原诱导的血小板聚集,但不抑制 PT 和 APTT,表明 AF-08 抑制血小板聚集但不抑制血液凝固。在AF-08所含的三种黄酮类化合物中,芹菜素和白杨素对血小板聚集有明显的抑制作用,而山奈酚的抑制作用较弱。这三种黄酮类化合物不影响 PT 和 APTT。AF-08 对人血小板聚集的抑制活性而不影响血液凝固被认为部分归因于芹菜素和白杨素。AF-08 可有效抑制基于血小板的动脉血栓形成并降低 CVD 风险。