Abstracts

In a model vegetation experiment (30 days), the responses of microbial communities of agro soddy-podzolic soil of two sites (Chashnikovo, Moscow oblast) with different organic carbon content (C_org 3.86 and 1.30%) to polymetallic pollution with heavy metals (HM) (Cu 660, Zn 1100, Pb 650 mg/kg) and treatment with biochar (5%) and lignohumate (0.25%). Classical microbiological culture-dependent analysis using Czapek agar and analysis of soil lipid profiles by gas chromatography–mass spectrometry revealed the differences in abundance colony forming units (CFUs), fungal and bacterial biomass, as well as in the diversity of cultivated fungi both in humus-rich and humus-poor soil. HMs did not have a significant effect on the number of CFUs and the number of cultural-morphological types of colonies, but reduced fungal and bacterial biomass in both soils; this decrease was more significant in a humus-poor soil. In addition, differences between the soils in the increase in the proportion of resistant melanized forms of fungi under the influence of HM in humus-rich (by 25.9%) and in humus-poor soil (by 45.7%) were observed. The sensitivity and universal significance of structural indicators as indicators of the stability of microbial complexes under chemical contamination of soils with different humus content were discussed. It was shown that the most sensitive and reliable indicators among the studied included the proportion of melanized fungi.

中文翻译：

---

多金属污染和土壤固结土壤修复中微生物复合物的结构

摘要

在一个模型植被实验（30天）中，两个有机碳含量不同的土壤（Chashnikovo，莫斯科州）的农业水田土壤土壤微生物群落的响应（C _org 3.86和1.30％）重金属（HM）（Cu 660，Zn 1100，Pb 650 mg / kg）的多金属污染，并用生物炭（5％）和木质素酸盐（0.25％）处理。使用Czapek琼脂进行经典的依赖于微生物培养的分析以及通过气相色谱-质谱法分析土壤脂质概况揭示了腐殖质中丰富菌落形成单位（CFU），真菌和细菌生物量以及栽培真菌多样性的差异富含腐殖质的土壤。HMs对CFU的数量和菌落的培养形态类型的数量没有显着影响，但是在两种土壤中真菌和细菌的生物量均减少了。这种减少在腐殖质贫瘠的土壤中更为明显。此外，在富含腐殖质的地区（减少了25.9％）和贫腐质的土壤中（减少了45.7％），在HM的影响下，土壤之间的抗性黑色素化形式真菌比例的增加之间存在差异。讨论了结构指标作为不同腐殖质含量的土壤化学污染下微生物复合物稳定性指标的敏感性和普遍意义。结果表明，所研究的最敏感，最可靠的指标包括黑色素化真菌的比例。