当前位置: X-MOL 学术Agron. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genome-wide association analysis of drought resistance based on seed germination vigor and germination rate at the bud stage in common bean
Agronomy Journal ( IF 2.1 ) Pub Date : 2021-05-03 , DOI: 10.1002/agj2.20683
Lei Wu 1, 2 , Yujie Chang 1, 2 , Lanfen Wang 1, 2 , Shumin Wang 1, 2 , Jing Wu 1, 2
Affiliation  

Common bean (Phaseolus vulgaris L.) is an important food legume. However, drought influences its entire growth and development process and can cause yield losses. The influence of drought stress is the most severe in the bud stage. The major objective of our study was to identify a series of loci involved in drought stress response in the bud stage using association analysis. In this study, 361 common bean germplasms were used to investigate germination vigor (GV) and germination rate (GR) under distilled water and drought stress conditions. The common bean showed large variations in GV and GR, with GV ranging from 6.67 to 100% and 0 to 73.33% and GR ranging from 66.67 to 100% and 0 to 100% in distilled water and under drought stress, respectively. A genome-wide association study (GWAS) of 361 accessions was conducted using 3,724,159 single nucleotide polymorphisms (SNPs) and revealed 2 and 11 candidate SNPs associated with relative germination rate (RGR) and relative germination vigor (RGV), respectively. Three loci were collocated with loci previously reported as being related to drought tolerance. A total of 180 candidate genes were identified, many of which were related to ion transport and oxidation-reduction reactions. A major haplotype for RGV was identified on chromosome 6. These results provide new insight into the genetic basis of drought tolerance in common bean at the bud stage and provide candidate SNPs and genes that can be used to accelerate the drought tolerance improvement of common bean.

中文翻译:

基于种子萌发力和芽期发芽率的普通豆抗旱性全基因组关联分析

普通豆 ( Phaseolus vulgaris)L.) 是一种重要的食物豆类。然而,干旱会影响其整个生长和发育过程,并可能导致产量损失。干旱胁迫的影响在芽期最为严重。我们研究的主要目的是使用关联分析确定一系列参与芽期干旱胁迫响应的位点。本研究利用361份常见豆类种质研究蒸馏水和干旱胁迫条件下的发芽势(GV)和发芽率(GR)。普通豆的 GV 和 GR 变化很大,在蒸馏水和干旱胁迫下,GV 范围分别为 6.67 至 100% 和 0 至 73.33%,GR 范围为 66.67 至 100% 和 0 至 100%。使用 3,724 种材料对 361 种材料进行了全基因组关联研究 (GWAS),159 个单核苷酸多态性 (SNP),分别揭示了 2 个和 11 个与相对萌发率 (RGR) 和相对萌发活力 (RGV) 相关的候选 SNP。三个基因座与先前报道的与耐旱性相关的基因座并置。共鉴定出180个候选基因,其中许多与离子转运和氧化还原反应有关。在第 6 号染色体上鉴定了 RGV 的一个主要单倍型。这些结果为了解普通豆芽期耐旱性的遗传基础提供了新的见解,并提供了可用于加速普通豆耐旱性提高的候选 SNP 和基因。三个基因座与先前报道的与耐旱性相关的基因座并置。共鉴定出180个候选基因,其中许多与离子转运和氧化还原反应有关。在第 6 号染色体上鉴定了 RGV 的一个主要单倍型。这些结果为了解普通豆芽期耐旱性的遗传基础提供了新的见解,并提供了可用于加速普通豆耐旱性提高的候选 SNP 和基因。三个基因座与先前报道的与耐旱性相关的基因座并置。共鉴定出180个候选基因,其中许多与离子转运和氧化还原反应有关。在第 6 号染色体上鉴定了 RGV 的一个主要单倍型。这些结果为了解普通豆芽期耐旱性的遗传基础提供了新的见解,并提供了可用于加速普通豆耐旱性提高的候选 SNP 和基因。
更新日期:2021-05-03
down
wechat
bug