当前位置: X-MOL 学术Proc. Natl. Acad. Sci. U.S.A. › 论文详情
Compliant 3D frameworks instrumented with strain sensors for characterization of millimeter-scale engineered muscle tissues [Engineering]
Proceedings of the National Academy of Sciences of the United States of America ( IF 9.412 ) Pub Date : 2021-05-11 , DOI: 10.1073/pnas.2100077118
Hangbo Zhao, Yongdeok Kim, Heling Wang, Xin Ning, Chenkai Xu, Judy Suh, Mengdi Han, Gelson J. Pagan-Diaz, Wei Lu, Haibo Li, Wubin Bai, Onur Aydin, Yoonseok Park, Jiaojiao Wang, Yao Yao, Yishan He, M. Taher A. Saif, Yonggang Huang, Rashid Bashir, John A. Rogers

Tissue-on-chip systems represent promising platforms for monitoring and controlling tissue functions in vitro for various purposes in biomedical research. The two-dimensional (2D) layouts of these constructs constrain the types of interactions that can be studied and limit their relevance to three-dimensional (3D) tissues. The development of 3D electronic scaffolds and microphysiological devices with geometries and functions tailored to realistic 3D tissues has the potential to create important possibilities in advanced sensing and control. This study presents classes of compliant 3D frameworks that incorporate microscale strain sensors for high-sensitivity measurements of contractile forces of engineered optogenetic muscle tissue rings, supported by quantitative simulations. Compared with traditional approaches based on optical microscopy, these 3D mechanical frameworks and sensing systems can measure not only motions but also contractile forces with high accuracy and high temporal resolution. Results of active tension force measurements of engineered muscle rings under different stimulation conditions in long-term monitoring settings for over 5 wk and in response to various chemical and drug doses demonstrate the utility of such platforms in sensing and modulation of muscle and other tissues. Possibilities for applications range from drug screening and disease modeling to biohybrid robotic engineering.



中文翻译:

装有应变传感器的兼容3D框架,用于表征毫米级工程肌肉组织[工程]

芯片组织系统代表了有前途的平台,可用于在体外监测和控制生物医学研究中各种目的的组织功能。这些构造的二维(2D)布局限制了可以研究的交互类型,并将它们与三维(3D)组织的相关性限制了。具有针对实际3D组织量身定制的几何形状和功能的3D电子支架和微生理设备的开发,有可能为高级感测和控制创造重要的可能性。这项研究提出了一系列符合标准的3D框架,这些框架结合了微型应变传感器,可在定量模拟的支持下对工程化的光遗传学肌肉组织环的收缩力进行高灵敏度测量。与基于光学显微镜的传统方法相比,这些3D机械框架和传感系统不仅可以测量运动,而且可以高精度和高时间分辨率测量收缩力。在超过5 wk的长期监测设置下,对不同刺激条件下的工程化肌肉环进行主动张力测量的结果,并响应各种化学和药物剂量,证明了此类平台在感测和调节肌肉及其他组织中的实用性。应用范围从药物筛选和疾病建模到生物杂交机器人工程。在超过5 wk的长期监测设置下,对不同刺激条件下的工程化肌肉环进行主动张力测量的结果,并响应各种化学和药物剂量,证明了此类平台在感测和调节肌肉及其他组织中的实用性。应用范围从药物筛选和疾病建模到生物杂交机器人工程。在超过5 wk的长期监测设置下,对不同刺激条件下的工程化肌肉环进行主动张力测量的结果,并响应各种化学和药物剂量,证明了此类平台在感测和调节肌肉及其他组织中的实用性。应用范围从药物筛选和疾病建模到生物杂交机器人工程。

更新日期:2021-05-03
全部期刊列表>>
专攻离子通道生理学研究
2021中国学者有奖调研
JACS
中国作者高影响力研究精选
虚拟特刊
屿渡论文,编辑服务
浙大
上海中医药大学
南方科技大学
舒伟
季恒星
毛凌玲
灵长脑研究中心
上海交大
朱如意
中科院
南开大学
隐藏1h前已浏览文章
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
华辉
天合科研
x-mol收录
试剂库存
down
wechat
bug