The expression of G2 and S phase-expressed-1 (GTSE1) was upregulated in human cancer. However, its expression and roles in lung cancer have not been identified yet. In our study, we reported that GTSE1 expression was statistically higher in lung tissues than in the adjacent noncancerous tissues which might be a consequence of hypomethylation of the GTSE1 promoter. The upregulated expression of GTSE1 mRNA predicted the poorer survival of the lung patients. Ectopic expression of GTSE1 in lung cancer cells significantly increased while knockdown of GTSE1 decreased cell proliferation, cell migration, and cell invasion in H460 and A549 cells. Furthermore, knockdown of GTSE1 regulated the cell cycle and promoted cell apoptosis in H460 and A549 cells. Finally, we presented that GTSE1 was able to activate AKT/mTOR signaling in H460 and A549 cells. In conclusion, these results indicated that the overexpressed GTSE1 was involved in the progress of lung cancer by promoting proliferation migration and invasion and inhibiting apoptosis of lung cancer cells via activating AKT/mTOR signaling.

中文翻译：

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GTSE1 通过激活 AKT/mTOR 信号促进肺癌细胞的恶性表型

G2 和 S 期表达-1 (GTSE1) 的表达在人类癌症中上调。然而，其在肺癌中的表达和作用尚未确定。在我们的研究中，我们报道了 GTSE1 在肺组织中的表达在统计学上高于相邻的非癌组织，这可能是 GTSE1 启动子低甲基化的结果。GTSE1 mRNA 的上调表达预示着肺患者的生存期较差。GTSE1 在肺癌细胞中的异位表达显着增加，而 GTSE1 的敲低降低了 H460 和 A549 细胞中的细胞增殖、细胞迁移和细胞侵袭。此外，GTSE1 的敲低调节细胞周期并促进 H460 和 A549 细胞的细胞凋亡。最后，我们提出 GTSE1 能够激活 H460 和 A549 细胞中的 AKT/mTOR 信号。