Objective

An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation.

Results

Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125 µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05).

Conclusion

Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.

中文翻译：

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超顺磁性锌镍铁氧体纳米颗粒标记人间充质干细胞对细胞特征和脂肪生成/成骨分化的影响

客观的

基于干细胞的治疗的一个有吸引力的细胞来源是 WJ-MSC。因此，需要使用非侵入性成像程序（如 MRI）和造影剂（Zn0.5Ni0.5Fe2O4、NFNPs）跟踪 WJ-MSC 以评估细胞分布、迁移和分化。

结果

结果表明，裸露和涂有糊精的 NFNPs 被内化在 WJ-MSCs 内，并且对 WJ-MSCs 的细胞活力、增殖、凋亡、核型分析和形态学无影响，浓度高达 125 µg/mL。此外，处理过的 WJ-MSC 分化为骨/脂肪细胞样细胞。的表达RUNX 2，SPP 1（P <0.05），和OCN（P> 0.05），其与糊精涂覆NFNPs处理的WJ-MSC的基因是比未处理的WJ-MSC的高; 与未处理的 WJ-MSC 相比，用两种 NFNP 处理的 WJ-MSC中CFD、LPL和PPAR-γ基因的表达降低（P > 0.05）。

结论

总体而言，结果表明糊精包覆的 NFNPs 对 WJ-MSCs 的细胞特征、增殖和分化没有不利影响，并表明其潜在的临床疗效。