当前位置: X-MOL 学术Physiol. Entomol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fluctuating thermal regimes extend longevity and maintain fecundity to increase shelf-life of Drosophila melanogaster cultures
Physiological Entomology ( IF 1.5 ) Pub Date : 2021-05-02 , DOI: 10.1111/phen.12357
Dacotah Melicher 1 , Amanda M. Wilson 2 , George D. Yocum 1 , Joseph P. Rinehart 1
Affiliation  

Reduced temperatures increase longevity in cold-tolerant insects, but insects that are not cold-tolerant experience elevated mortality at constant low temperatures. Fluctuating thermal regimes (FTRs) increase longevity in many insect species while slowing or delaying development and senescence. Under FTR insects are held at low temperature with a daily warm pulse of increased temperature. The model organism Drosophila melanogaster is widely used for diverse research activities and numerous transgenic strains have been developed and must be maintained in continuous culture at stock centres. We measured the effect of FTR that oscillates between 6 and 22 °C on the longevity and fertility of adult D. melanogaster versus a constant temperature of 6 and 22 °C. We demonstrate that FTR increases mean longevity by 8.8-fold compared to a constant 6 °C and by 5.9-fold to a constant 22 °C. We assessed male and female fertility of FTR treated adults from 20 to 100 days at 20 day intervals and constant temperature treated adults after 7 days. Under FTR males exhibited increased fertility peaking at 80 days before dropping significantly at 100 days. Mean female fertility declined steadily under FTR but remained at 53% of constant temperature-treated flies after 60 days. The increased male fertility remains unexplained. Fertility of the offspring of FTR-treated adults did not differ from flies reared at constant 22 °C. FTR extends generations and cultures incubated under this protocol can establish new cultures. FTR can be used to further investigate the accumulation and mitigation of chill injury and is suitable for reducing maintenance in stock centres.

中文翻译:

波动的热状态延长寿命并保持繁殖力,以增加黑腹果蝇培养物的保质期

降低温度会增加耐寒昆虫的寿命,但不耐寒的昆虫在恒定低温下会增加死亡率。波动的热状态 (FTR) 增加了许多昆虫物种的寿命,同时减缓或延迟了发育和衰老。在 FTR 下,昆虫被保持在低温下,每天都有温度升高的暖脉冲。模式生物黑腹果蝇被广泛用于各种研究活动,并且已经开发了许多转基因菌株,并且必须在库存中心连续培养。我们测量了在 6 到 22 °C 之间振荡的 FTR 对成年D的寿命和生育能力的影响。黑腹果蝇与 6 和 22 °C 的恒定温度相比。我们证明,与恒定 6 °C 相比,FTR 将平均寿命增加 8.8 倍,与恒定 22 °C 相比增加 5.9 倍。我们每隔 20 天评估 FTR 治疗成人 20 至 100 天的男性和女性生育能力,并在 7 天后评估恒温治疗成人的男性和女性生育能力。在 FTR 下,雄性的生育能力在 80 天达到峰值,然后在 100 天显着下降。在 FTR 下,平均雌性生育力稳步下降,但在 60 天后保持在恒温处理果蝇的 53%。男性生育能力的增加仍然无法解释。经 FTR 处理的成虫后代的生育力与在恒定 22°C 下饲养的果蝇没有差异。FTR 扩展了世代,在该协议下培养的文化可以建立新的文化。
更新日期:2021-05-02
down
wechat
bug