Biopesticides such as essential oils (EOs) are considered an improvement for integrated pest control as they appear to be less toxic to the environment than chemical acaricides. The current study aimed to evaluate the acaricidal activity of Artemisia herba-alba and Melia azedarach oil loaded nano-emulsion as alternatives for chemical acaricides against the camel tick Hyalomma dromedarii, besides evaluating their toxic effect on Swiss albino mice. Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) were used for the characterization of loaded nano-emulsions.The immersion test was used for the bioassay of both loaded nanoemulsions on tick stages (egg, nymph, larva, and adult). Mortality percentages and LC₅₀ values of each tick stage were calculated. Reproductive performance for the survived engorged females after treatment was monitored. The toxicity of both loaded nano-emulsions was evaluated on Swiss albino mice by an oral dose of 1500 mg/kg/day for five successive days. The hematological, biochemical, and histopathological changes were evaluated. TEM characterization revealed spherical droplets for A. herba-alba and M. azedarach oil loaded nano-emulsion with droplet size ranging from 62 to 69 nm and 52–91 nm, respectively. FTIR revealed the absence of extra peaks in the loaded nano-emulsions that confirmed no chemical changes existed by ultrasonication. The LC₅₀ values of A. herba-alba and M. azedarach oil loaded nano-emulsion on embryonated eggs, larvae, engorged nymphs, and unfed adults were 0.3 and 1.1%, 0.7 and 1.7%, 0.3 and 0.4%, 4.4 and 22.2%, respectively. The egg productive index (EPI), egg number, and hatchability percentage were lower in the treated females compared with Butox 5% (deltamethrin) and control. The hematological picture and biochemical analysis revealed insignificant changes in the treatment group compared with the negative control group. The liver of the A. herba-alba and M. azedarach oil loaded nano-emulsion treated group exhibited vacuolar degeneration and infiltration of lymphocytic cells. The kidney of mice treated with A. herba-alba and M. azedarach oil loaded nano-emulsion showed hemolysis and slight degeneration of epithelial cells of tubules. It is concluded that A. herba-alba and M. azedarach oil loaded nano-emulsion have good acaricidal activity against camel tick H. dromedarii.

生物农药（例如精油（EOs））被认为是病虫害综合防治的一种改进，因为它们对环境的毒性似乎小于化学杀螨剂。当前的研究旨在评估青蒿油和Mel子油载纳米乳液作为化学杀螨剂的替代品对骆驼虱Hyalomma dromedarii的杀螨活性，同时评估其对瑞士白化病小鼠的毒性作用。使用透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）表征负载的纳米乳液，浸没测试用于在滴答阶段（例如卵，若虫，幼虫和成虫）对两种负载的纳米乳液进行生物测定。 。死亡率和LC ₅₀计算每个刻度阶段的值。监测治疗后存活的饱食的雌性的生殖性能。连续五天以1500 mg / kg / day的口服剂量在瑞士白化病小鼠上评估了两种负载的纳米乳剂的毒性。评估了血液学，生化和组织病理学变化。TEM表征显示球形油滴对A.herba-alba和M. azedarach油负载的纳米乳状液的液滴大小分别为62至69 nm和52-91 nm。FTIR显示在负载的纳米乳液中没有多余的峰，这证实了超声处理不存在化学变化。A.herba-alba和M.azedarach的LC ₅₀值胚卵，幼虫，若虫，若虫和未摄食的成虫上含油纳米乳剂分别为0.3和1.1％，0.7和1.7％，0.3和0.4％，4.4和22.2％。与5％的Butox（溴氰菊酯）和对照组相比，处理过的雌性的产蛋指数（EPI），卵数和孵化率较低。血液学图像和生化分析显示，与阴性对照组相比，治疗组的变化不明显。载有A.herba-alba和M. azedarach油的纳米乳处理组的肝脏表现出液泡变性和淋巴细胞的浸润。用草曲霉和阿兹达拉赫菌治疗的小鼠肾脏载油纳米乳液表现出溶血和肾小管上皮细胞轻微变性。可以得出结论，载有A. herba-alba和M. azedarach油的纳米乳剂对骆驼虱dromedarii具有良好的杀螨活性。