Illumination conditions are crucial factors affecting the growth and biosynthesis of microalgae. In this study, monochromatic red, blue, or the combined red and blue light illumination was applied to Dunaliella salina culture to study the effects of illumination on the growth and biocomponents productivity of the microalga D. salina. The monochromatic blue light (peak wavelength, 455 nm) did not significantly enhance D. salina growth but boosted the cellular contents of lipid, protein, and carbohydrate. Both cellular content and productivity of carotenoid produced by D. salina in the white light control were the highest compared to other monochromatic treatments. Interestingly, simultaneous application of red and blue light in various ratios showed a major boost not only on growth but also on cellular lipid content, and resulted in higher lipid productivity. This result is different from many published literatures reporting the enhancement of biomass or cellular lipid content individually. The optimal ratio of red and blue light was 4:3 (4 dose of red light to 3 dose of blue light), which significantly improved lipid productivity by 35.33% compared to the white light control. The result suggested a monochromatic red and blue light combination of illumination could be employed to increase the biomass yield as well as cellular lipid content of D. salina simultaneously for the production of biofuel.

中文翻译：

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组合单色光对盐沼杜氏藻生物成分生产力的影响

光照条件是影响微藻生长和生物合成的关键因素。在这项研究中，将单色的红色，蓝色或红色和蓝色的组合光照应用于杜氏盐藻培养，以研究光照对微藻盐藻生长和生物组分生产力的影响。单色蓝光（峰值波长为455 nm）没有显着增强盐藻的生长，但增加了脂质，蛋白质和碳水化合物的细胞含量。盐藻产生的类胡萝卜素的细胞含量和生产力与其他单色处理相比，白光控制中的最高。有趣的是，同时施加不同比例的红光和蓝光不仅对生长而且对细胞脂质含量都显示出极大的促进作用，并导致更高的脂质生产率。该结果与许多公开报道的文献有所不同，后者分别报告了生物量或细胞脂质含量的增加。红光和蓝光的最佳比例为4：3（4剂量的红光对3剂量的蓝光），与白光对照相比，其脂质生产率显着提高了35.33％。结果表明，可以采用单色红色和蓝色光组合照明来提高盐生杜氏藻的生物量产量和细胞脂质含量。 同时用于生产生物燃料。