ABSTRACT

In soybean, studies on drought-responses are conducted during vegetative season. Information related to genes triggered in response to water-deficit (WD) in flower and pod is lacking. We performed an RNASeq and an agro-physiological characterization at stages R2 and R4 of soybean cultivar BR16, under WD. Physiological results showed a decrease in gas exchange parameters. Agronomical results showed WD impaired yield. Global Gene Ontology analyses indicated that most of the Differential Expressed Genes (DEGs) were down-regulated in flowers but up-regulated in pods. qRT-PCR revealed that WD triggered hormone biosynthesis changes. Mechanisms such as a decrease in glyceollin levels and cell wall instability were observed. This data showed tissue-specific mechanisms correlated with phenotypic responses such as drought escape, early flowering hormone-induced, and loss in grain weight. We presented here a comprehensive expression profile of flowers and pods soybean genes, which could guide researchers in the development of plants more tolerant to drought.

摘要

在大豆中，在营养季节进行了干旱响应研究。缺乏与花和豆荚中的缺水（WD）响应而触发的基因相关的信息。在WD条件下，我们在大豆品种BR16的R2和R4阶段进行了RNASeq和农业生理学表征。生理结果显示气体交换参数降低。农艺结果表明WD减产。全球基因本体论分析表明，大多数差异表达基因（DEG）在花中被下调，但在豆荚中被上调。qRT-PCR显示WD触发了激素的生物合成变化。观察到了诸如甘油分子水平降低和细胞壁不稳定性的机制。该数据显示与表型反应相关的组织特异性机制，例如干旱逃逸，早期开花激素诱导，并失去谷粒重量。我们在这里介绍了花和豆荚大豆基因的全面表达谱，这可以指导研究人员开发更耐干旱的植物。