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Whole-Genome Sequence Analysis of Italian Honeybees (Apis mellifera)
Animals ( IF 3 ) Pub Date : 2021-05-02 , DOI: 10.3390/ani11051311
Giulietta Minozzi , Barbara Lazzari , Maria Grazia De Iorio , Cecilia Costa , Emanuele Carpana , Paola Crepaldi , Rita Rizzi , Elena Facchini , Gustavo Gandini , Alessandra Stella , Giulio Pagnacco

At the end of the last glaciation, Apis mellifera was established in northern Europe. In Italy, Apis melliferaligustica adapted to the mild climate and to the rich floristic biodiversity. Today, with the spread of Varroa destructor and with the increasing use of pesticides in agriculture, the Ligustica subspecies is increasingly dependent on human action for its survival. In addition, the effects of globalization of bee keeping favored the spread in Italy of other honeybee stocks of A. mellifera, in particular the Buckfast bee. The purpose of this study was to characterize the Italian honeybee’s population by sequencing the whole genome of 124 honeybees. Whole genome sequencing was performed by Illumina technology, obtaining a total coverage of 3720.89X, with a mean sample coverage of 29.77X. A total of 4,380,004 SNP variants, mapping on Amel_HAv3.1 chromosomes, were detected. Results of the analysis of the patterns of genetic variation allowed us to identify and subgroup bees according to their type. The investigation revealed the genetic originality of the Sicula, and in A.m. ligustica limited genetic introgression from the other breeds. Morphometric analysis of 5800 worker bees was in agreement with genomic data.

中文翻译:

意大利蜜蜂全基因组序列分析

在最后一次冰期结束时,Apis mellifera在北欧成立。在意大利,蜜蜂Apis mellifera ligustica适应了温和的气候和丰富的植物生物多样性。如今,随着Varroa破坏性病毒的传播以及农药在农业中的使用日益增加,女贞属亚种的生存越来越依赖于人类的行动。此外,养蜂全球化的影响也促进了其他蜜蜂种群A. mellifera在意大利的传播,尤其是Buckfast蜜蜂。这项研究的目的是通过对124个蜜蜂的整个基因组进行测序来表征意大利蜜蜂的种群。全基因组测序是通过Illumina技术进行的,总覆盖率为3720.89X,平均样本覆盖率为29.77X。共检测到4,380,004个SNP变体,定位在Amel_HAv3.1染色体上。遗传变异模式的分析结果使我们能够根据蜜蜂的类型对其进行鉴定和分组。调查揭示了Sicula的遗传独创性,并在上午ligustica从其他品种的限制遗传渗入。5800只工蜂的形态计量学分析与基因组数据一致。
更新日期:2021-05-03
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