A composite chiral interface (BSA-MB-MWCNTs) was prepared from bovine serum albumin (BSA), methylene blue (MB), and multi-walled carbon nanotubes (MWCNTs) for chiral recognition of amine enantiomers (1S, 2S)-N,N′-dimethyl-1,2-cyclohexanediamine and (1R, 2R)-N,N′-dimethyl-1,2-cyclohexanediamine. The BSA-based composite was characterized by field emission scanning electron microscopy (FESEM) and ultraviolet–visible spectroscopy (UV–Vis). The electrochemical responses towards the two enantiomers were analyzed via cyclic voltammetry (CV), electrochemical AC impedance method (EIS), and differential-pulse voltammetry (DPV). The experimental results showed that the combination of MWCNTs and BSA could effectively improve the overall identification efficiency, and the peak current displayed by the S-enantiomer is larger, indicating that the prepared chiral surface has stronger interaction with the R-enantiomer. Under optimized condition, the current value of the oxidation peak of the chiral modified electrode showed a good linear relationship towards the amine concentration in the range of 5.0 × 10⁻³ to 5.0 × 10⁻⁵ mmol·L⁻¹. The proposed electrochemical chiral interface is easy to handle and provides a promising electrochemical sensing platform that can be used to identify chiral amine enantiomers.

中文翻译：

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基于BSA的生物分子手性界面用于胺对映异构体的电化学识别

由牛血清白蛋白 (BSA)、亚甲蓝 (MB) 和多壁碳纳米管 (MWCNTs) 制备复合手性界面 (BSA-MB-MWCNTs)，用于手性识别胺对映异构体 (1 S , 2 S )- N , N '-二甲基-1,2-环己二胺和 (1 R , 2 R )- N , N'-二甲基-1,2-环己二胺。基于 BSA 的复合材料通过场发射扫描电子显微镜 (FESEM) 和紫外-可见光谱 (UV-Vis) 进行表征。通过循环伏安法 (CV)、电化学交流阻抗法 (EIS) 和差分脉冲伏安法 (DPV) 分析了对两种对映异构体的电化学响应。实验结果表明，MWCNTs与BSA的结合能有效提高整体识别效率，S-对映体显示的峰值电流更大，表明制备的手性表面与R的相互作用更强。-对映体。在优化条件下，手性修饰电极氧化峰的电流值在5.0×10 ^-3至5.0×10 ^-5  mmol·L ^-1范围内与胺浓度呈良好的线性关系。所提出的电化学手性界面易于处理，并提供了一个有前途的电化学传感平台，可用于识别手性胺对映体。