Strategies to improve hydroxyapatite mineralization for enamel repair are essential for tissue regeneration. Amelogenin and amelotin (AMTN) are enamel matrix proteins playing critical roles in enamel formation. Amelogenin acts as a scaffold for hydroxyapatite, while AMTN (specifically its ‘SSEEL’ domain) is necessary for proper enamel mineralization. The functional relationship between recombinant AMTN and amelogenin, and their combined ability to guide uniaxial hydroxyapatite growth in vitro has been investigated recently. However, incorporation of the active domain of AMTN within recombinant amelogenins has not been studied yet. Here we describe the synthesis of modified amelogenin by inserting, internally and at its C-terminus, the mineralizing AMTN-derived motif SSEEL. C-terminus modified amelogenin promoted hydroxyapatite formation, whereas internal incorporation of the motif initially resulted in amorphous calcium phosphate formation. Here we show that modified amelogenin with the mineralizing AMTN-motif SSEEL can promote hydroxyapatite growth. These results give new insights for mineralized tissue regeneration using recombinamer proteins.

中文翻译：

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牙釉蛋白-牙釉蛋白重组体上羟基磷灰石的生长

改善用于牙釉质修复的羟基磷灰石矿化的策略对于组织再生至关重要。牙釉蛋白和牙釉蛋白 (AMTN) 是牙釉质基质蛋白，在牙釉质形成中起关键作用。牙釉蛋白作为羟基磷灰石的支架，而 AMTN（特别是它的“SSEEL”域）是正确的牙釉质矿化所必需的。重组 AMTN 和牙釉蛋白之间的功能关系，以及它们在体外指导单轴羟基磷灰石生长的联合能力最近被调查了。然而，尚未研究在重组牙釉蛋白中掺入 AMTN 的活性结构域。在这里，我们通过在内部和 C 端插入矿化 AMTN 衍生的基序 SSEEL 来描述修饰的牙釉蛋白的合成。C-末端修饰的牙釉蛋白促进了羟基磷灰石的形成，而该基序的内部掺入最初导致无定形磷酸钙的形成。在这里，我们表明具有矿化 AMTN 基序 SSEEL 的改性牙釉蛋白可以促进羟基磷灰石的生长。这些结果为使用重组体蛋白的矿化组织再生提供了新的见解。