The production of the black soldier fly (BSF), Hermetia illucens L. (Diptera: Stratiomyidae), has potential to promote sustainable food production, and is environmentally friendly through organic waste upcycling. The objective of this study was to determine the digestive enzyme activities and chemical composition of BSF larvae of 6, 12, and 18 days old at various substrate pH and temperature conditions during ontogenic development. Cellulase and chymotrypsin-like protease (C) activities declined slightly with age, whereas a sharp increase in lipase activity was found with age. High levels of amylase, trypsin-like protease (T), trehalase, and T/C ratio were present in 12-day-old BSF, with a gradual decrease in 18-day-old BSF. The highest crude protein and lipid levels were observed at 6 and 18 days of age, respectively. In conclusion, the activities of the various types of enzymes illustrate the polyphagous nature of BSF. Most of the digestive enzymes had high activity at an early age and then dropped at the end of the larval stage; however, the opposite trend was observed in lipase activity, with a high level of fat accumulation in the last stage of larval development.

中文翻译：

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不同年龄Hermetia illucens幼虫消化酶和化学成分的个体发育

黑水虻(BSF) Hermetia illucens 的制作L.（双翅目：Stratiomyidae）具有促进可持续粮食生产的潜力，并且通过有机废物升级回收对环境友好。本研究的目的是确定在个体发育过程中在各种底物 pH 和温度条件下 6、12 和 18 天龄的 BSF 幼虫的消化酶活性和化学成分。纤维素酶和胰凝乳蛋白酶样蛋白酶 (C) 活性随着年龄的增长略有下降，而脂肪酶活性随着年龄的增长而急剧增加。12 天龄的 BSF 中存在高水平的淀粉酶、胰蛋白酶样蛋白酶 (T)、海藻糖酶和 T/C 比值，而 18 天龄的 BSF 则逐渐减少。分别在 6 日龄和 18 日龄时观察到最高的粗蛋白和脂质水平。综上所述，各种酶的活性说明了 BSF 的多食性。大部分消化酶在幼年时期活性很高，在幼虫期末期活性下降；然而，在脂肪酶活性中观察到相反的趋势，在幼虫发育的最后阶段脂肪积累水平很高。