Cryptocaryon irritans is a parasitic ciliate that causes cryptocaryonosis (white spot disease) in marine fish. Because the pathogenesis of cryptocaryonosis is unclear, and it is harmful to aquaculture, the identification of reliable biomarkers as a specific therapy for the disease is critical. To identify potential serum biomarkers in Takifugu rubripes infected by C. irritans, Agilent-QTOF/MS-6545 mass spectrometry-based untargeted metabolite profiling was applied to differentiate and identify potential biomarkers in blood serum samples we collected from 10 fish infected by C. irritans and 10 healthy controls. The results of untargeted metabolic pathway analysis showed that retinol metabolism, steroid hormone biosynthesis, purine metabolism, arachidonic acid metabolism and drug metabolism involving cytochrome P450 were significantly different between infected and control groups. Based on data from the receiver operating characteristic curve, we propose that retinoic acid, progesterone, xanthosine monophosphate, hepoxilin B3, tamoxifen and prostaglandin I2 are possible potential biomarkers for infected fish in terms of both sensitivity and specificity. Our study provides new insight into the underlying mechanisms of T. rubripes infected by C. irritans, and we suggest that metabolic pathways and metabolites identified in our work could make a major contribution to the prevention and treatment of this disease in aquaculture.

中文翻译：

---

代谢组学分析用于鉴定受白点虫攻击的 Takifugu rubripes 血清中的潜在生物标志物

Cryptocaryon irritans是一种寄生纤毛虫，可导致海鱼隐核病（白点病）。由于隐核病的发病机制尚不清楚，并且对水产养殖有害，因此鉴定可靠的生物标志物作为该疾病的特异性治疗方法至关重要。要识别潜在的血清标志物红鳍东方鲀的传染Ç。irtans，基于 Agilent-QTOF/MS-6545 质谱的非靶向代谢物分析被应用于区分和鉴定我们从 10 条被C感染的鱼收集的血清样本中的潜在生物标志物。刺激物和 10 个健康对照。非靶向代谢途径分析结果显示，感染组与对照组的视黄醇代谢、类固醇激素生物合成、嘌呤代谢、花生四烯酸代谢和涉及细胞色素P450的药物代谢有显着差异。根据接收者操作特征曲线的数据，我们建议视黄酸、黄体酮、单磷酸黄苷、hepoxilin B3、他莫昔芬和前列腺素 I2 可能是受感染鱼的潜在生物标志物，无论是敏感性还是特异性。我们的研究为T的潜在机制提供了新的见解。被C感染的rubripes。刺激物，我们建议在我们的工作中确定的代谢途径和代谢物可以为水产养殖中这种疾病的预防和治疗做出重大贡献。