Habitual chewing of areca nut increases the risk of cardiovascular disease mortality, but less report demonstrate the toxic mechanism of areca nut on heart. To investigate toxicity of areca nut on cardiomyocytes, we induced the heart injury with arecoline to evaluate the acute damage of areca nut on heart. Different concentrations of are coline (lowdosage: 5 mg/kg/day and high dosage 50 mg/kg/day) were injected into Sprague-Dawley rat via intra-peritoneal method for 21 days to create negative effects of arecoline on cardiomyocyte. Themyocardial architecture of the rat heart was observed. The arecoline-induced apoptotic proteins were analysed via western blotting. The myocardialarchitecture of heart was injured with arecoline and TUNEL stain was also shown are coline-induced cardiac apoptosis. Arecoline promoted the protein expression of both Fas dependent snd mitochondrial dependent apoptosis. In summary, arecoline induces cardiac toxicity and apoptosis by inducing both death receptor and mitochondria-dependent apoptotic pathways on heart.

中文翻译：

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槟榔碱通过 Sprague-Dawley 大鼠心脏中的 Fas/Fas 配体凋亡途径诱导心脏损伤

习惯性咀嚼槟榔会增加心血管疾病死亡风险，但很少有报道证明槟榔对心脏的毒性机制。为了研究槟榔对心肌细胞的毒性，我们用槟榔碱诱导心脏损伤，以评估槟榔对心脏的急性损伤。将不同浓度的槟榔碱（低剂量：5mg/kg/天和高剂量：50mg/kg/天）通过腹膜内方法注射到 Sprague-Dawley 大鼠体内 21 天，以产生槟榔碱对心肌细胞的负面影响。观察大鼠心脏的心肌结构。通过蛋白质印迹分析槟榔碱诱导的凋亡蛋白。槟榔碱损伤了心脏的心肌结构，TUNEL 染色也显示为coline 诱导的心脏细胞凋亡。槟榔碱促进Fas依赖性和线粒体依赖性细胞凋亡的蛋白质表达。总之，槟榔碱通过诱导心脏上的死亡受体和线粒体依赖性细胞凋亡途径来诱导心脏毒性和细胞凋亡。