Abstract

In mammalian cells, base excision repair (BER) is the main pathway responsible for the correction of a variety of chemically modified DNA bases. DNA packaging in chromatin affects the accessibility of damaged sites to the enzymes involved in repair processes. This review presents data concerning the enzymes involved in BER. Within the nucleosome core particle (NCP), the accessibility of damaged DNA to enzymes is hindered by the presence of a histone octamer. This means that the removal of DNA lesions largely depends on their rotational and translational positioning in the NCP, as well as on the specific features of each enzyme.

中文翻译：

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核小体中DNA碱基切除修复的初始阶段

摘要

在哺乳动物细胞中，碱基切除修复（BER）是负责校正各种化学修饰的DNA碱基的主要途径。染色质中的DNA包装会影响受损位点对修复过程中涉及的酶的可及性。这篇综述提供了有关BER中涉及的酶的数据。在核小体核心颗粒（NCP）中，组蛋白八聚体的存在阻碍了受损DNA对酶的可及性。这意味着DNA损伤的去除很大程度上取决于它们在NCP中的旋转和翻译位置，以及每种酶的特定特征。